Laboratoire de Cytophysiologie de la Photosynthèse, Centre National de la Recherche Scientifique, F-91190, Gif-sur-Yvette, France.
Planta. 1979 Jan;145(3):259-67. doi: 10.1007/BF00454450.
Dark-grown, DCMU-adapted Euglena gracilis Z (ZR) are able to undergo light-induced chloroplast development in the presence or absence of DCMU. The differentiated chloroplasts are photosynthetically active and are resistant not only to DCMU, but also to an analog, o-phenanthrolene. When DCMU overdoses are added to ZR cells or to chloroplasts isolated from these cells, photosynthesis is partially inhibited. A brief period of darkness removes this inhibition. This recovery phenomenon is related to DCMU resistance, since it is not exhibited by non-resistant control cells. The chloroplast protein synthesis apparatus is not involved in DCMU resistance. Rather, this phenomenon is apparently related to new characteristics of thylakoids. It is shown that photosynthetic recovery by ZR cells depends on the accessibility and fluid properties of membranes. The analysis of fluorescence induction kinetics shows that changes in the environmental conformation of photosystem II units occur during recovery.
黑暗生长、DCMU 适应的绿眼虫 Z（ZR）在有或没有 DCMU 的情况下都能进行光诱导叶绿体发育。分化的叶绿体具有光合作用活性，不仅对 DCMU 有抗性，而且对类似物邻菲罗啉也有抗性。当向 ZR 细胞或从这些细胞中分离的叶绿体中添加 DCMU 过量时，光合作用会受到部分抑制。短暂的黑暗会消除这种抑制。这种恢复现象与 DCMU 抗性有关，因为非抗性对照细胞不会表现出这种现象。叶绿体蛋白合成装置不参与 DCMU 抗性。相反，这种现象显然与类囊体的新特性有关。结果表明，ZR 细胞的光合作用恢复取决于膜的可及性和流体特性。荧光诱导动力学分析表明，在恢复过程中发生了光系统 II 单位环境构象的变化。
