Botany School, South Parks Road, OX1 3RA, Oxford.
Planta. 1979 Jan;145(5):411-6. doi: 10.1007/BF00380094.
Caffeine, (1:3:7-tri-methyl-xanthine), either as a prefixation treatment or included with glutaralde-hyde as the primary fixative, destroys or disorganises the microtubules associated with the formation of secondary walls in fibres from the flowering stem of the grass Lolium temulentum L. There is no observable effect of caffeine treatment on the microtubules associated with primary wall formation in collenchyma and young fibres from L. temulentum or in root cap cells of Zea mays L. and Phaseolus vulgaris L. The microtubules associated with primary wall formation are destroyed by cold treatment but not those associated with secondary wall formation. Tannic acid included in the fixative shows the microtubules associated with secondary wall formation in fibres of L. temulentum to be composed of 13 subunits. Treatment with lanthanum hydroxide does not stain the core or the halo of the microtubules.
咖啡因（1：3：7-三甲基黄嘌呤），无论是作为预处理还是与戊二醛一起作为主要固定剂，都会破坏或打乱与次级细胞壁形成相关的微管，这些微管存在于 Lolium temulentum L. 的开花茎纤维中。在 Lolium temulentum 的厚角组织和年轻纤维以及 Zea mays L. 和 Phaseolus vulgaris L. 的根帽细胞中，与初生壁形成相关的微管没有观察到咖啡因处理的影响。冷处理会破坏与初生壁形成相关的微管，但不会破坏与次生壁形成相关的微管。固定剂中包含的单宁酸表明，与次生壁形成相关的微管由 13 个亚基组成。用氢氧化镧处理不会染色微管的核心或晕圈。
