USDA-ARS National Forage Seed Production Research Center, 3450 SW Campus Way, Corvallis, OR 97331, USA.
J Plant Physiol. 2012 Jun 15;169(9):915-9. doi: 10.1016/j.jplph.2012.03.003. Epub 2012 Apr 1.
Forage and turf grasses are utilized in diverse environments that expose them to a variety of abiotic stresses, however very little is known concerning the perception or molecular responses to these various stresses. In the model grass species Lolium temulentum, a 46kDa mitogen-activated protein kinase (MAPK) was activated in the leaf within 10min of exposing the roots to salt stress. When plants were subjected cold stress, no significant activation of the MAPK was observed. However, the 46kDa MAPK was rapidly activated in the leaves of plants within 3min of exposure to heat stress. Previously, mechanical wounding has been shown to rapidly activate a 46kDa and a 44kDa MAPK in L. temulentum. The wound activation of the MAPKs was delayed and diminished in plants undergoing cold treatment. In plants subjected simultaneously to 40°C and wounding, the activation of the 46kDa MAPK was enhanced. However if plants were subjected to heat and cold stress for more than 2h or exposed to 300mM NaCl for 24h prior to wounding, the wound activation of the 46kDa and a 44kDa MAPKs were significantly inhibited. These results suggest that the 46kDa MAPK plays a role in the response to various environmental stimuli.
饲料和草坪草被用于多种环境中，这些环境使它们暴露于各种非生物胁迫下，但对于这些不同胁迫的感知或分子响应知之甚少。在模式草本植物多花黑麦草中，根暴露于盐胁迫后 10 分钟内，46kDa 丝裂原活化蛋白激酶（MAPK）在叶片中被激活。当植物受到冷胁迫时，未观察到 MAPK 的明显激活。然而，当植物受到热胁迫时，46kDa MAPK 在 3 分钟内迅速在叶片中被激活。先前已经表明，机械损伤会迅速激活多花黑麦草中的 46kDa 和 44kDa MAPK。在进行冷处理的植物中，MAPKs 的伤口激活被延迟和减弱。在同时受到 40°C 和伤口处理的植物中，46kDa MAPK 的激活增强。然而，如果植物在受伤前受到超过 2 小时的热和冷胁迫，或者暴露于 300mM NaCl 24 小时，则 46kDa 和 44kDa MAPKs 的伤口激活会受到显著抑制。这些结果表明，46kDa MAPK 在响应各种环境刺激中起作用。
