Hume W J, Saffhill R
Chem Biol Interact. 1986 Nov;60(2):227-32. doi: 10.1016/0009-2797(86)90031-1.
The development of antibodies to iododeoxyuridine (IdU) and bromodeoxyuridine (BrdU) has increased interest in the use of these compounds in cell biology. However, little is known about their metabolism in vivo and in vitro. In this paper we show that, contrary to the impression gained from earlier studies, these compounds are not stably incorporated into DNA of mouse tongue epithelial cells in vitro but are removed, with turnover times of 1.0 days (BrdU) and 3.8 days (IdU).
针对碘脱氧尿苷（IdU）和溴脱氧尿苷（BrdU）的抗体的开发，增加了人们对在细胞生物学中使用这些化合物的兴趣。然而，对于它们在体内和体外的代谢情况却知之甚少。在本文中，我们表明，与早期研究所得到的印象相反，这些化合物在体外并不会稳定地掺入小鼠舌上皮细胞的DNA中，而是会被去除，其周转时间分别为1.0天（BrdU）和3.8天（IdU）。
