5-卤代脱氧尿苷对转化的大鼠膀胱上皮细胞系体外角化的抑制作用。

Inhibitory effect of 5-halogenated deoxyuridines on in vitro keratinization of a transformed rat urinary bladder epithelial cell line.

作者信息

Kawamura H, Hashimoto Y

出版信息

Gan. 1981 Apr;72(2):264-71.

Abstract

The effect of 5-halogenated deoxyuridines on the keratinization of an in vitro-transformed rat bladder epithelial cell line, BES20B, was studied by a cover slip culture method established previously. 5-Bromodeoxyuridine (BrdU) and 5-iododeoxyuridine (IdU) both showed dose-dependent inhibition of the keratinization. The complete inhibition of keratinization was achieved by BrdU at concentrations higher than 5 microgram/ml. At these concentrations, BrdU reduced the saturation density of the test cells on a cover slip. Removal of BrdU from the culture medium restored the cell growth as well as the keratinization of BrdU-treated cells, suggesting that the effect of BrdU is reversible. The effect of BrdU was abolished by the addition of excess thymidine. A possible mechanism of the anti-keratinization effect of BrdU is discussed in comparison with that of vitamin A.

摘要

采用先前建立的盖玻片培养法，研究了5-卤代脱氧尿苷对体外转化的大鼠膀胱上皮细胞系BES20B角质化的影响。5-溴脱氧尿苷（BrdU）和5-碘脱氧尿苷（IdU）均表现出剂量依赖性的角质化抑制作用。当浓度高于5微克/毫升时，BrdU可完全抑制角质化。在这些浓度下，BrdU降低了盖玻片上测试细胞的饱和密度。从培养基中去除BrdU可恢复细胞生长以及BrdU处理细胞的角质化，这表明BrdU的作用是可逆的。添加过量胸苷可消除BrdU的作用。与维生素A相比，讨论了BrdU抗角质化作用的可能机制。