Chemical Engineering Area, Department of Engineering, University of Almería, 04120, Almería, Spain.
Appl Microbiol Biotechnol. 2014 Mar;98(6):2429-40. doi: 10.1007/s00253-013-5413-9. Epub 2013 Dec 7.
Different pilot-scale outdoor photobioreactors using medium recycling were operated in a greenhouse under different environmental conditions and the growth rates (0.1 to 0.5 day(-1)) obtained evaluated in order to compare them with traditional systems used in aquaculture. The annualized volumetric growth rate for Nannochloropsis gaditana was 0.26 g l(-1) day(-1) (peak 0.4 g l(-1) day(-1)) at 0.4 day(-1) in a 5-cm wide flat-panel bioreactor (FP-PBR). The biomass productivity achieved in this reactor was 10-fold higher than in traditional reactors, reaching values of 28 % and 45 % dry weight (d.w.) of lipids and proteins, respectively, with a 4.3 % (d.w.) content of eicosapentaenoic acid (EPA). A model for predicting EPA productivity from N. gaditana cultures that takes into account the existence of photolimitation and photoinhibition of growth under outdoor conditions is presented. The effect of temperature and average irradiance on EPA content is also studied. The maximum EPA productivity attained is 30 mg l(-1) day(-1).
不同的中水循环式户外光生物反应器在不同的环境条件下在温室中运行，并对获得的生长率（0.1 至 0.5 天(-1))进行了评估，以便将其与水产养殖中使用的传统系统进行比较。在 0.4 天(-1)的情况下，在 5 厘米宽的平板光生物反应器（FP-PBR）中，Nannochloropsis gaditana 的年化体积生长率为 0.26 g l(-1) day(-1)（峰值为 0.4 g l(-1) day(-1))。在这种反应器中实现的生物量生产力比传统反应器高 10 倍，分别达到 28%和 45%的干重（d.w.）脂质和蛋白质，其中二十碳五烯酸（EPA）的含量为 4.3%（d.w.）。提出了一种从 N. gaditana 培养物中预测 EPA 生产力的模型，该模型考虑了户外条件下光限制和生长光抑制的存在。还研究了温度和平均辐照度对 EPA 含量的影响。达到的最大 EPA 生产力为 30 mg l(-1) day(-1)。
