University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Bioresour Technol. 2018 Apr;253:1-7. doi: 10.1016/j.biortech.2017.12.102. Epub 2017 Dec 30.
In this study, Nannochloropsis oceanica CY2 was grown in deep-sea water (DSW)-based medium in 5-L plastic bag-type photobioreactors (PBRs) for the autotrophic production of Eicosapentaenoic acid (EPA, 20:5n-3). EPA production of N. oceanica CY2 was stimulated when it was grown in 100% DSW amended with 1.5 g L NaNO, achieving a EPA content of 3.1% and a biomass concentration of 3.3 g L. An outdoor-simulated microalgae cultivation system was also conducted to validate the feasibility of outdoor cultivation of the CY2 strain in plastic bag-type PBRs. Using an inoculum size of 0.6 g/L, the biomass concentration in the PBR culture was 3.5 g L, while the EPA content and productivity reached a maximal level of 4.12% and 7.49 mg L d, respectively. When the PBRs were operated on semi-batch mode, the EPA productivity could further increase to 9.9 mg L d with a stable EPA content of 4.1%.
在这项研究中，使用深海海水（DSW）为基础的培养基，在 5 升塑料袋式光生物反应器（PBR）中培养海洋盐藻（Nannochloropsis oceanica）CY2，用于二十碳五烯酸（EPA，20:5n-3）的自养生产。当 CY2 在 100% DSW 中生长，并添加 1.5 g/L 的 NaNO3 时，其 EPA 产量得到刺激，EPA 含量达到 3.1%，生物量浓度达到 3.3 g/L。还进行了户外模拟微藻培养系统，以验证 CY2 菌株在塑料袋式 PBR 中进行户外培养的可行性。使用 0.6 g/L 的接种量，PBR 培养物中的生物量浓度达到 3.5 g/L，而 EPA 含量和生产力分别达到最高水平 4.12%和 7.49 mg/L/d。当 PBR 以半分批模式运行时，EPA 生产力可进一步提高至 9.9 mg/L/d，同时保持稳定的 EPA 含量 4.1%。
