Forschungsstelle Vennesland der Max-Planck-Gesellschaft, Harnackstraße 23, D-1000, Berlin 33, Germany.
Planta. 1979 Oct;146(5):539-44. doi: 10.1007/BF00388829.
Added vanadate ions inhibit purified nitrate reductase from Chlorella vulgaris by reacting with the enzyme in a manner rather similar to that of HCN. Thus vanadate, like HCN, forms an inactive complex with the reduced enzyme, and this inactivated enzyme can be reactivated rapidly by adding ferricyanide. The inactive vanadate enzyme complex is less stable than the inactive HCN complex, and the two can be distinguished by the fact that EDTA causes a partial reactivation of the former, but not of the latter. Vanadate can also cause an increase in HCN formation by intact Chlorella vulgaris cells. When these cells were incubated with vanadate, their nitrate reductase was reversibly inactivated, and all of this inactive enzyme could be shown to be the HCN complex rather than the vanadate complex. When HCN and vanadate are both present, the HCN-inactivated enzyme, being more stable, will be formed in preference to the vanadate-inactivated enzyme.
添加的钒酸盐离子通过与酶发生反应来抑制来自普通小球藻的纯化硝酸还原酶，其方式与 HCN 相当相似。因此，钒酸盐与 HCN 一样，与还原酶形成无活性的复合物，而这种失活的酶可以通过添加铁氰化物迅速重新激活。失活的钒酸盐酶复合物不如失活的 HCN 复合物稳定，并且可以通过 EDTA 引起前者的部分重新激活而不是后者来区分这两种复合物。钒酸盐还可以通过完整的普通小球藻细胞增加 HCN 的形成。当这些细胞与钒酸盐一起孵育时，它们的硝酸还原酶可逆地失活，并且可以证明所有失活的酶都是 HCN 复合物而不是钒酸盐复合物。当同时存在 HCN 和钒酸盐时，更稳定的 HCN 失活酶将优先形成而不是钒酸盐失活酶。
