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用亲和层析法纯化 NADH-硝酸盐还原酶。

Purification of NADH-Nitrate Reductase by Affinity Chromatography.

机构信息

Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242.

出版信息

Plant Physiol. 1975 Dec;56(6):853-5. doi: 10.1104/pp.56.6.853.

DOI:10.1104/pp.56.6.853
PMID:16659410
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC541940/
Abstract

ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of mumolar concentrations of NADH.

摘要

小球藻同化硝酸还原酶（NADH：硝酸氧化还原酶，EC 1.6.6.1）已通过使用蓝色葡聚糖-琼脂糖作为亲和柱的程序被纯化至电泳均一性，总产率为 60％。硝酸还原酶与蓝色葡聚糖结合，并且在存在高离子强度缓冲液的情况下不会被洗脱，但在存在毫摩尔浓度 NADH 的情况下会迅速被洗脱。

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引用本文的文献

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Pyridine nucleotide specificity and other properties of purified nitrate reductase from Chlorella variegata.从衣藻中纯化的硝酸还原酶的吡啶核苷酸特异性和其他性质。
Planta. 1979 Jan;144(2):137-41. doi: 10.1007/BF00387262.
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Planta. 1979 Oct;146(5):539-44. doi: 10.1007/BF00388829.
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本文引用的文献

1
Activation of nitrate reductase by oxidation.通过氧化作用激活硝酸还原酶。
Biochim Biophys Acta. 1972 Aug 17;275(2):276-8. doi: 10.1016/0005-2728(72)90048-5.
2
Further purification and properties of Neurospora nitrate reductase.粗糙脉孢菌硝酸还原酶的进一步纯化及性质
J Biol Chem. 1969 Jun 10;244(11):2870-82.
3
Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach.从大鼠肝脏和肝癌组织中快速纯化乳酸脱氢酶：一种新方法。
Arch Biochem Biophys. 1974 Jan;160(1):279-84. doi: 10.1016/s0003-9861(74)80035-4.
4
Properties of a nitrate reductase of Chlorella.小球藻硝酸还原酶的特性
Biochim Biophys Acta. 1972 Jun 23;267(3):544-57. doi: 10.1016/0005-2728(72)90183-1.
5
[Reciprocity of yeast-phosphofructokinase with dextran blue 2000].[酵母磷酸果糖激酶与葡聚糖蓝2000的相互作用]
Eur J Biochem. 1971 Sep 13;22(1):40-5. doi: 10.1111/j.1432-1033.1971.tb01512.x.
6
Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.蓝色葡聚糖-琼脂糖凝胶：一种用于蛋白质中二核苷酸折叠结构的亲和柱。
Proc Natl Acad Sci U S A. 1975 Feb;72(2):669-72. doi: 10.1073/pnas.72.2.669.