Department of Agricultural Chemistry, Washington State University, 99164, Pullman, WA, USA.
Planta. 1979 Oct;146(5):607-14. doi: 10.1007/BF00388840.
Combined gas chromatography-mass spectrometry showed that C21, C23, and C25 n-alkanes accumulated in the suberized layers during wound healing of cores of potato tuber tissue. Treatment (10 min) of freshly-cut tissue with trichloroacetate (TCA), an inhibitor of fatty-acid chain elongation, severely inhibited accumulation of hydrocarbons and fatty alcohols associated with the suberized layer in the wound healing tissue (maximum inhibition at 4 mM) but had very little effect on the deposition of the major aliphatic components of the suberin polymer. This preferential inhibition of wax synthesis resulted in severe inhibition of the development of diffusion resistance of the tissue to water vapor. These results strongly indicate that the waxes associated with the suberin polymer, rather than the polymer itself, consitute the major diffusion barrier formed during wound healing. Electron-microscopic examination showed that inhibition of wax synthesis by TCA disrupted the formation of the lamellar structure of suberin specifically by preventing the formation of the light bands. This evidence strongly suggests that the light bands in the suberin complex are composed of waxes.
联合气相色谱-质谱分析表明，在马铃薯块茎组织芯伤口愈合过程中，C21、C23 和 C25 正构烷烃在木栓化层中积累。用三氯乙酸（TCA）处理（10 分钟）新切组织，TCA 是脂肪酸链延长的抑制剂，严重抑制了与伤口愈合组织中木栓化层相关的碳氢化合物和脂肪醇的积累（在 4mM 时达到最大抑制），但对木栓质聚合物的主要脂肪族成分的沉积几乎没有影响。这种蜡合成的优先抑制导致组织对水蒸气扩散阻力的发展严重抑制。这些结果强烈表明，与木栓质聚合物相关的蜡，而不是聚合物本身，构成了伤口愈合过程中形成的主要扩散屏障。电子显微镜检查表明，TCA 对蜡合成的抑制作用通过阻止轻带的形成而特异性地破坏了木栓质的层状结构的形成。这一证据强烈表明，木栓质复合物中的亮带由蜡组成。
