Zellenlehre, Universität Heidelberg, Im Neuenheimer Feld 230, D-6900, Heidelberg, Federal Republic of Germany.
Planta. 1979 Oct;146(5):615-21. doi: 10.1007/BF00388841.
With chlorotetracycline (CTC)-fluorescence a tip-to-base Ca(2+) gradient is visualized in all tested, tip-growing plant cells: pollen tubes of Lilium longiflorum, root hairs of Lepidium sativum, moss caulonema of Funaria hygrometrica, fungal hyphae of Achlya and in the alga Acetabularia mediterranea. The fluorescence gradients in the different species vary in intensity and extension. Sometimes a punctate mobile CTC-fluorescence, in the size range of mitochondria, is observed. Bursting cells lose their fluorescence rapidly, indicating a cytoplasmic localization of the gradient. Only in Acetabularia is the wall also fluorescent with CTC. The results are interpreted as evidence for a general role of a calcium gradient in tip growth.
用氯四环素（CTC）荧光标记，在所有测试的顶端生长的植物细胞中都可以观察到从顶到基的 Ca（2+）梯度：百合花粉管、生菜根毛、湿地藓蒴帽状茎、水绵和地中海蓝藻。不同物种的荧光梯度在强度和延伸上有所不同。有时会观察到点状的、线粒体大小的、可移动的 CTC 荧光。爆裂的细胞迅速失去荧光，表明梯度定位于细胞质。只有在地中海蓝藻中，细胞壁也用 CTC 荧光标记。这些结果被解释为钙梯度在顶端生长中普遍起作用的证据。
