Augsburger Heinz R, Führer Constanze
Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland,
Int Urogynecol J. 2014 May;25(5):657-62. doi: 10.1007/s00192-013-2275-8. Epub 2013 Dec 7.
Urinary incontinence is prevalent in postmenopausal women and spayed dogs and is associated with decreased estrogen plasma concentrations. The objective of the study was to investigate the expression of estrogen receptors (ER) in the urethra of sexually intact, ovariectomized, and estrogen-substituted ovariectomized ewes.
Paraffin cross-sections from each urethral quarter were immunohistochemically analyzed. The reactivity of ER was semiquantitatively assessed employing an immunoreactive score (IRS).
In contrast to ERβ, ERα was identified in all urethral compartments; the highest IRS was detected in the epithelium of the distal urethra. The immunoreactivity and distribution of ERα did not differ among groups. Highly significant differences in ERα concentrations were observed between consecutive urethral quarters in each group.
Neither ovariectomy nor ovariectomy and estrogen substitution seem to have a significant effect on overall urethral ERα concentration. The results demonstrate that the precise location of the investigated urethral part is crucial to the reliable evaluation or possible comparison of ERα concentrations.
尿失禁在绝经后女性和绝育犬中普遍存在，且与血浆雌激素浓度降低有关。本研究的目的是调查性成熟、去卵巢和雌激素替代去卵巢母羊尿道中雌激素受体（ER）的表达。
对每个尿道象限的石蜡切片进行免疫组织化学分析。采用免疫反应评分（IRS）对ER的反应性进行半定量评估。
与ERβ不同，ERα在所有尿道区域均有发现；在尿道远端上皮中检测到最高的IRS。各组之间ERα的免疫反应性和分布没有差异。在每组中，连续的尿道象限之间观察到ERα浓度存在高度显著差异。
去卵巢以及去卵巢加雌激素替代似乎对尿道整体ERα浓度均无显著影响。结果表明，所研究尿道部位的精确位置对于可靠评估或比较ERα浓度至关重要。
