孕酮受体在小鼠下尿路中的性别特异性表达。

Sex specific expression of progesterone receptor in mouse lower urinary tract.

作者信息

Savolainen Saija, Santti Risto, Streng Tomi, Gustafsson Jan-Ake, Härkönen Pirkko, Mäkelä Sari

机构信息

Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland.

出版信息

Mol Cell Endocrinol. 2005 Jan 31;230(1-2):17-21. doi: 10.1016/j.mce.2004.11.008.

DOI:10.1016/j.mce.2004.11.008

PMID:15664447

Abstract

Progesterone receptor (PR) was investigated immunohistochemically in the lower urinary tract of the male and female mouse. Estrogen receptor (ER)-subtype-deficient mice (ERKO, BERKO) were used to determine the possible regulation of PR expression in an ER-subtype-specific manner. PR was found to be co-expressed with ERalpha in cell nuclei of urothelium, lamina propria fibroblasts and smooth muscle cells in the female urethra. Only few PR positive cells were seen in female ERKO mice. Ovariectomy reduced and estrogen treatment restored the urethral PR expression in female wild type and BERKO mice. Thus, the expression of PR in the female urethra is estrogen-inducible via ERalpha. In male urethra, PR was co-expressed with ERbeta in the rhabdosphincter. In male, no evidence was obtained for the ER-linked control of the PR expression. No PR-positive cells were observed in the body of the bladder of either sex or any strain.

摘要

采用免疫组织化学方法研究了雄性和雌性小鼠下尿路中的孕激素受体（PR）。利用雌激素受体（ER）亚型缺陷小鼠（ERKO、BERKO）以ER亚型特异性方式确定PR表达的可能调控机制。发现PR在雌性尿道的尿路上皮、固有层成纤维细胞和平滑肌细胞的细胞核中与ERα共表达。在雌性ERKO小鼠中仅见少数PR阳性细胞。卵巢切除降低了雌性野生型和BERKO小鼠尿道PR的表达，而雌激素处理可使其恢复。因此，雌性尿道中PR的表达可通过ERα由雌激素诱导。在雄性尿道中，PR在横纹括约肌中与ERβ共表达。在雄性中，未获得PR表达受ER相关调控的证据。在任何性别或品系的膀胱体部均未观察到PR阳性细胞。