用正相高效液相色谱法定量测定光合系统 I 颗粒中的叶绿素 a'和叶绿醌。
Normal-phase HPLC quantitation of chlorophyll a' and phylloquinone in Photosystem I particles.
机构信息
Institute of Industrial Science, University of Tokyo, Roppongi, Minato-ku, 106, Tokyo, Japan.
出版信息
Photosynth Res. 1993 Feb;35(2):179-84. doi: 10.1007/BF00014748.
Fractionated Photosystem (PS) I particles consisting of six, five or two core proteins were analyzed by HPLC for chlorophyll (Chl) a' and phylloquinone (PhQ). Each particle had a Chl a/P700 molar ratio of 50-55 and contained ca. 2 molecules of Chl a' per P700. Deliberate control of eluent composition led to isolated elution of PhQ and β-carotene in the normal-phase chromatogram. Based on these a simple HPLC procedure has been established to determine the PhQ/P700 molar ratio, which was ca. 2 for the larger two PS I particles and ca. 1 for the smallest particle, in line with previous reports.
采用 HPLC 分析了由六个、五个或两个核心蛋白组成的分段光系统 I（PS I）颗粒中的叶绿素（Chl）a'和叶绿醌（PhQ）。每个颗粒的 Chl a/P700 摩尔比为 50-55，每个 P700 含有约 2 个 Chl a'分子。通过控制洗脱液组成，可以在正相色谱图中分离出 PhQ 和β-胡萝卜素。基于这些结果，建立了一种简单的 HPLC 方法来测定 PhQ/P700 摩尔比，对于较大的两个 PS I 颗粒，其摩尔比约为 2，对于最小的颗粒，其摩尔比约为 1，与之前的报道一致。
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