Larman Mark G, Gardner David K
Department of Zoology, The University of Melbourne, Melbourne, VIC, Australia.
Methods Mol Biol. 2014;1092:153-65. doi: 10.1007/978-1-60327-292-6_10.
Cryopreservation facilitates long-term storage of gametes and embryos for numerous purposes. For example, cryobanking of unique mouse strains, particularly transgenic mice, offers important protection of valuable genetics. It also provides a practical solution for facilities trying to house large numbers of research animals or those looking to relocate without the risk of introducing an animal-derived pathogen. Furthermore, cryopreservation is currently being used for fertility preservation both in humans and as a safeguard for endangered animals. Ultrarapid vitrification offers an elegant, quick, and very reliable method for cryopreservation of mouse oocytes and embryos. Furthermore, research into the effects on mouse oocyte and embryo physiology has indicated that ultrarapid vitrification is superior to conventional slow freezing. High survival rates, embryo development, and viability are routinely achieved with the ultrarapid vitrification method described in this chapter.
冷冻保存有助于出于多种目的对配子和胚胎进行长期储存。例如，对独特的小鼠品系，特别是转基因小鼠进行冷冻保存，可为珍贵的基因提供重要保护。它还为试图饲养大量实验动物的设施或那些希望搬迁而无引入动物源性病原体风险的设施提供了切实可行的解决方案。此外，冷冻保存目前正用于人类的生育力保存以及作为濒危动物的一种保护措施。超快速玻璃化提供了一种优雅、快速且非常可靠的小鼠卵母细胞和胚胎冷冻保存方法。此外，对小鼠卵母细胞和胚胎生理学影响的研究表明，超快速玻璃化优于传统的慢速冷冻。采用本章所述的超快速玻璃化方法通常可实现高存活率、胚胎发育和活力。
