Department of Biology, Lafayette College, 18042, Easton, Pennsylvania.
J Chem Ecol. 1984 Jul;10(7):1065-9. doi: 10.1007/BF00987513.
Since free sterol excretory-secretory (E-S) products are involved in pheromonal communication in adultEchinostoma revolutum (Trematoda), attempts were made to associate specific systems with the release of lipids from this organism. A micropipet design was used to isolate neutral lipids from the excretory system versus those obtained from both the alimentary and the reproductive systems. Tegumentary lipids were obtained by rubbing the surface of worms with gauze. As determined by thin-layer chromatography, the major neutral lipid obtained from all systems was free sterol. Additional minor neutral lipid fractions were obtained from the excretory, alimentary, and reproductive systems. Histochemical oil red O studies showed neutral lipids only in the excretory system. Neutral lipids released from all of the above-mentioned systems may play a role in pheromonal communication in this species.
由于游离固醇排泄-分泌（E-S）产物参与了成年异形吸虫（吸虫纲）的信息素通讯，因此试图将特定系统与该生物释放脂质联系起来。使用微吸管设计从排泄系统中分离中性脂质，与从消化道和生殖系统中获得的脂质进行分离。通过用纱布摩擦虫体来获得表皮脂质。通过薄层色谱法确定，从所有系统中获得的主要中性脂质是游离固醇。从排泄系统、消化道和生殖系统中还获得了其他少量的中性脂质。组织化学油红 O 研究表明，只有在排泄系统中才有中性脂质。从上述所有系统释放的中性脂质可能在该物种的信息素通讯中发挥作用。
