Badraoui Riadh, Boubakri Mariem, Bedbabiss Maissa, Ben-Nasr Hmed, Rebai Tarek
Laboratory of Histo-Embryology and Cytogenetic, Medicine Faculty, University of Sfax, 3029, Sfax, Tunisia,
Tumour Biol. 2014 Apr;35(4):3663-70. doi: 10.1007/s13277-013-1485-5. Epub 2013 Dec 8.
This study was designed to assess femur angioarchitecture and hematological effects of Walker 256/B cells in a rat model of tumor osteolysis. Tumor osteolysis was induced by in situ inoculation of Walker 256/B malignant cells. Six other rats were sham operated and served as control. Twenty days later, rats were euthanized, and femurs were collected than radiographed. Angioarchitecture [mean lumen diameter (MLD), wall thickness (WTh), Vessel number, volume, and separation (VNb, VV, and VSp respectively)] was studied by histomorphometry at 2 different positions (P1: diaphysis, and P2: metaphysis) of the operated femora. Some hematological parameters were also assessed. Walker 256/B induced marked tumor osteolysis, with cortical perforation and trabecular destruction, associated increase in bone vascularization (increases of VNb and VV and decrease of VSp). Angioarchitecture of W256/B rats was disorganized and showed large MLD and lower WTh. These effects were more prominent in P2. When compared to Sham group, significantly decreases at levels of red blood cell (RBC), hemoglobin (Hb), hematocrit (Ht), and white blood cell (WBC) were observed in W256/B rats. These results suggest that Walker 256/B cells induced tumor osteolysis, improve hypervasculature especially near the tumoral foci (P2) associated hematological disruption. Besides, tumor vessels showed abnormal (enlarged and thinner) and disorganized morphology.
本研究旨在评估Walker 256/B细胞在大鼠肿瘤性骨溶解模型中对股骨血管构筑和血液学的影响。通过原位接种Walker 256/B恶性细胞诱导肿瘤性骨溶解。另外6只大鼠接受假手术并作为对照。20天后，对大鼠实施安乐死，收集股骨并进行X线摄影。通过组织形态计量学研究手术侧股骨2个不同位置（P1：骨干，P2：干骺端）的血管构筑[平均管腔直径（MLD）、壁厚（WTh）、血管数量、体积和间距（分别为VNb、VV和VSp）]。还评估了一些血液学参数。Walker 256/B诱导明显的肿瘤性骨溶解，伴有皮质穿孔和小梁破坏，同时骨血管化增加（VNb和VV增加，VSp降低）。W256/B大鼠的血管构筑紊乱，表现为MLD大、WTh低。这些影响在P2更为显著。与假手术组相比，W256/B大鼠的红细胞（RBC）、血红蛋白（Hb）、血细胞比容（Ht）和白细胞（WBC）水平显著降低。这些结果表明，Walker 256/B细胞诱导肿瘤性骨溶解，改善了特别是肿瘤灶附近（P2）的血管过度增生，并伴有血液学紊乱。此外，肿瘤血管显示形态异常（增大且变薄）和紊乱。
