将自然杀伤细胞应用于临床：体外操作。
Bringing natural killer cells to the clinic: ex vivo manipulation.
作者信息
Childs Richard W, Berg Maria
机构信息
1Section of Transplantation Immunotherapy, Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD.
出版信息
Hematology Am Soc Hematol Educ Program. 2013;2013(1):234-46. doi: 10.1182/asheducation-2013.1.234.
Abstract
Recently, there has been a substantial gain in our understanding of the role that natural killer (NK) cells play in mediating innate host immune responses against viruses and cancer. Although NK cells have long been known to be capable of killing cancer cells independently of antigen recognition, the full therapeutic potential of NK cell-based immunotherapy has yet to be realized. Here we review novel methods to activate and expand human NK cells ex vivo for adoptive transfer in humans, focusing on the important phenotypic and functional differences observed among freshly isolated, cytokine activated, and ex vivo-expanded NK populations.
摘要
最近，我们对自然杀伤（NK）细胞在介导宿主针对病毒和癌症的先天性免疫反应中所起的作用有了更深入的了解。尽管长期以来人们都知道NK细胞能够独立于抗原识别来杀伤癌细胞，但基于NK细胞的免疫疗法的全部治疗潜力尚未实现。在此，我们综述了离体激活和扩增人NK细胞以用于人体过继转移的新方法，重点关注在新鲜分离的、细胞因子激活的和离体扩增的NK细胞群体中观察到的重要表型和功能差异。
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