Yamagami S, Onishi H, Ohno K, Mori K, Kawakita Y
Exp Neurol. 1987 Jan;95(1):167-77. doi: 10.1016/0014-4886(87)90015-x.
We examined the effects of convulsive seizures on in vitro RNA synthesis by cerebral cortex nuclei in El mice. The rate of incorporation of [3H]uridine-5'-triphosphate by intact nuclei during seizures was decreased to 47.4% compared with the rate during the interictal period, but gradually recovered. During the 30-min period after onset of seizures, the rate of RNA synthesis was significantly lower in El mice than in identically stimulated ddY mice. Seizures in El mice had no effect on liver RNA synthesis, suggesting that the alteration of RNA polymerase activity is specific to the brain. Analysis of gel electrophoresis of polyadenylated RNA synthesized in the presence of ammonium sulphate revealed a marked decrease in high-molecular weight RNA species 15 min after seizures in El mice compared with the pattern in nonstimulated ddY mice. This shift from high- to low-molecular weight RNA species was not attributable to RNase activity, but it appeared to be related RNA polymerase.
我们研究了惊厥发作对El小鼠大脑皮质细胞核体外RNA合成的影响。与发作间期相比，惊厥发作期间完整细胞核对[3H]尿苷-5'-三磷酸的掺入率降至47.4%，但随后逐渐恢复。惊厥发作开始后的30分钟内，El小鼠的RNA合成速率显著低于受到相同刺激的ddY小鼠。El小鼠的惊厥发作对肝脏RNA合成没有影响，这表明RNA聚合酶活性的改变是大脑特有的。对在硫酸铵存在下合成的聚腺苷酸化RNA进行凝胶电泳分析发现，与未受刺激的ddY小鼠相比，El小鼠惊厥发作15分钟后高分子量RNA种类显著减少。这种从高分子量RNA种类向低分子量RNA种类的转变并非归因于核糖核酸酶活性，而是似乎与RNA聚合酶有关。
