Wang Yu-Xue, Liu Wei, Tan Xin-Yu, Tang Hui-Huan
Department of Emergency Medicine, Xiang Ya Hospital, Central South University, Changsha 410008, China.
JRSM Short Rep. 2013 Sep 13;4(10):2042533313476690. doi: 10.1177/2042533313476690. eCollection 2013.
Perineural invasion of cholangiocarcinoma happens in the early stage of the disease but is often not recognized until its later stages. Research about the behaviour and mechanism of perineural invasion by cholangiocarcinoma is urgently needed for a useful new model. The aim of this work is to establish a novel model to address the problem.
Neural cells and cholangiocarcinoma cells were co-cultured to mimic the neurotropic invasion of cholangiocarcinoma.
Human embryonic stem cells were induced to form neural cells by glial cell-derived neurotropic factor and retinoic acid; neural cells and cholangiocarcinoma cells were co-cultured in Transwell chamber.
Human embryonic stem cells and cholangiocarcinoma cells were applied.
Paired t-test was used to compare the counts of penetrating cholangiocarcinoma cells in co-culture and control group.
Formation of neurospheres and neural-like cells were observed following induction at 24 and 48 h, respectively; synapses were viewed to protrude from neural-like cell bodies after incubation for 96 h. Forty-eight hours after incubation, immunocytochemical staining of the cells showed that synaptophysin and glial fibrillary acidic protein were expressed in the neuron-like cells and gliocytes-like cells, respectively. The cholangiocarcinoma cells that had penetrated through the Matrigel/polyethylene terephthalate membrane from the upper chamber to the lower chamber of the Transwell in the co-culture group were significantly more numerous than those in the control group (68 ± 8.3/field versus 46 ± 5.7/field, P < 0.05).
The novel model is a valuable tool to study the perineural invasion of cholangiocarcinoma.
胆管癌的神经周围浸润在疾病早期就会发生，但往往直到晚期才被发现。迫切需要研究胆管癌神经周围浸润的行为和机制，以建立一个有用的新模型。这项工作的目的是建立一个新模型来解决这个问题。
将神经细胞和胆管癌细胞共培养，以模拟胆管癌的嗜神经侵袭。
通过胶质细胞源性神经营养因子和视黄酸诱导人胚胎干细胞形成神经细胞；神经细胞和胆管癌细胞在Transwell小室中共培养。
应用人胚胎干细胞和胆管癌细胞。
采用配对t检验比较共培养组和对照组中穿透胆管癌细胞的数量。
分别在诱导24小时和48小时后观察到神经球和神经样细胞的形成；培养96小时后，可见突触从神经样细胞体中突出。培养48小时后，细胞免疫细胞化学染色显示，突触素和胶质纤维酸性蛋白分别在神经元样细胞和胶质细胞样细胞中表达。共培养组中从Transwell上室穿过基质胶/聚对苯二甲酸乙二酯膜进入下室的胆管癌细胞明显多于对照组（68±8.3/视野对46±5.7/视野，P<0.05）。
该新模型是研究胆管癌神经周围浸润的有价值工具。
