Urabe Kazuhide, Murakami Yoshiaki, Kondo Naru, Uemura Kenichiro, Hashimoto Yasushi, Nakagawa Naoya, Sasaki Hayato, Hiyama Eiso, Takahashi Shinya, Sueda Taijiro
Department of Surgery, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Natural Science Center for Basic Research and Development, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Dig Dis Sci. 2016 Mar;61(3):774-84. doi: 10.1007/s10620-015-3953-9. Epub 2015 Nov 7.
Although the presence of perineural invasion has been recognized as a poor prognostic factor in extrahepatic cholangiocarcinoma, the molecular mechanisms of perineural invasion in extrahepatic cholangiocarcinoma remain unclear. Nerve growth factor has been reported to be a candidate predictive biomarker of perineural invasion in some cancers.
To investigate the impact of intratumoral nerve growth factor expression in resected extrahepatic cholangiocarcinoma on survival.
Intratumoral nerve growth factor expression was investigated immunohistochemically in 112 patients with resected extrahepatic cholangiocarcinoma. Associations between nerve growth factor expression and clinicopathological factors were statistically evaluated, and risk factors for poor survival were analyzed using univariate and multivariate analyses.
High and low nerve growth factor expression was observed in 62 (55%) and 50 (45%) patients, respectively. For all 112 patients, no significant correlation was found between nerve growth factor expression and presence of perineural invasion (P = 0.942). Moreover, nerve growth factor expression was not associated with recurrence-free survival (P = 0.861) and overall survival (P = 0.973). In multivariate analysis, lymph node metastasis (P = 0.004) was identified as an independent risk factor for early recurrence and the presence of perineural invasion (P = 0.002) and lymph node metastasis (P < 0.001) was identified as independent risk factors for poor survival.
Intratumoral nerve growth factor expression is not associated with perineural invasion or recurrence-free and overall survival in patients with resected extrahepatic cholangiocarcinoma.
尽管神经周围浸润的存在已被认为是肝外胆管癌的不良预后因素，但肝外胆管癌神经周围浸润的分子机制仍不清楚。据报道，神经生长因子是某些癌症中神经周围浸润的候选预测生物标志物。
探讨切除的肝外胆管癌肿瘤内神经生长因子表达对生存的影响。
对112例接受肝外胆管癌切除术的患者进行免疫组织化学检测，以研究肿瘤内神经生长因子的表达情况。对神经生长因子表达与临床病理因素之间的相关性进行统计学评估，并采用单因素和多因素分析方法分析生存不良的危险因素。
分别在62例（55%）和50例（45%）患者中观察到高和低神经生长因子表达。在所有112例患者中，神经生长因子表达与神经周围浸润的存在之间未发现显著相关性（P = 0.942）。此外，神经生长因子表达与无复发生存期（P = 0.861）和总生存期（P = 0.973）无关。在多因素分析中，淋巴结转移（P = 0.004）被确定为早期复发的独立危险因素，神经周围浸润的存在（P = 0.002）和淋巴结转移（P < 0.001）被确定为生存不良的独立危险因素。
在接受肝外胆管癌切除术的患者中，肿瘤内神经生长因子表达与神经周围浸润、无复发生存期和总生存期无关。
