Department of Pharmacy, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki, Japan.
FEBS Open Bio. 2013 Nov 28;4:18-24. doi: 10.1016/j.fob.2013.11.004. eCollection 2013.
Mast cells have been suggested to play key roles in adipogenesis. We herein show that the expression of preadipocyte, but not adipocyte, marker genes increases in the white adipose tissue of mast cell-deficient (Kit(W-sh/W-sh) ) mice under both obese and non-obese conditions. In vitro culturing with adipogenic factors revealed increased adipocytes differentiated from the Kit(W-sh/W-sh) stromal vascular fraction, suggesting the accumulation of preadipocytes. Moreover, the increased expression of preadipocyte genes was restored by mast cell reconstitution in the Kit(W-sh/W-sh) mice. These results suggest positive effects of mast cells on the preadipocyte to adipocyte transition under both physiological and pathological conditions.
肥大细胞被认为在脂肪生成中发挥关键作用。我们在此表明，在肥胖和非肥胖条件下，脂肪细胞缺陷型（Kit(W-sh/W-sh)）小鼠的白色脂肪组织中，前脂肪细胞标志物基因的表达增加，而脂肪细胞标志物基因的表达则没有增加。用脂肪生成因子进行体外培养显示，来自 Kit(W-sh/W-sh)基质血管部分的脂肪细胞分化增加，提示前脂肪细胞的积累。此外，在 Kit(W-sh/W-sh)小鼠中，通过肥大细胞重建恢复了前脂肪细胞基因的高表达。这些结果表明，肥大细胞在生理和病理条件下对前脂肪细胞向脂肪细胞的转化有积极作用。
