Pollution Control Research Laboratory, Department of Chemical Engineering, Annamalai University, Annamalai Nagar, Tamil Nadu 608 002, India.
Biomed Res Int. 2013;2013:265618. doi: 10.1155/2013/265618. Epub 2013 Nov 11.
Mangrove sediments host rich assemblages of microorganisms, predominantly mixed bacterial cultures, which can be efficiently used for biohydrogen production through anaerobic dark fermentation. The influence of process parameters such as effect of initial glucose concentration, initial medium pH, and trace metal (Fe(2+)) concentration was investigated in this study. A maximum hydrogen yield of 2.34, 2.3, and 2.6 mol H2 mol(-1) glucose, respectively, was obtained under the following set of optimal conditions: initial substrate concentration-10,000 mg L(-1), initial pH-6.0, and ferrous sulphate concentration-100 mg L(-1), respectively. The addition of trace metal to the medium (100 mg L(-1) FeSO4 ·7H2O) enhanced the biohydrogen yield from 2.3 mol H2 mol(-1) glucose to 2.6 mol H2 mol(-1) glucose. Furthermore, the experimental data was subjected to kinetic analysis and the kinetic constants were estimated with the help of well-known kinetic models available in the literature, namely, Monod model, logistic model and Luedeking-Piret model. The model fitting was found to be in good agreement with the experimental observations, for all the models, with regression coefficient values >0.92.
红树林沉积物中蕴藏着丰富的微生物群落，主要是混合细菌培养物，通过厌氧暗发酵可以有效地用于生物制氢。本研究考察了初始葡萄糖浓度、初始培养基 pH 值和痕量金属（Fe(2+)）浓度等工艺参数的影响。在以下最佳条件下，分别获得了 2.34、2.3 和 2.6 mol H2/mol 葡萄糖的最大氢气产量：初始底物浓度-10,000 mg/L、初始 pH 值-6.0 和硫酸亚铁浓度-100 mg/L。向培养基中添加痕量金属（100 mg/L FeSO4·7H2O）将生物制氢产量从 2.3 mol H2/mol 葡萄糖提高到 2.6 mol H2/mol 葡萄糖。此外，对实验数据进行了动力学分析，并借助文献中可用的知名动力学模型（即 Monod 模型、逻辑斯蒂模型和 Luedeking-Piret 模型）估算了动力学常数。对于所有模型，模型拟合与实验观察结果非常吻合，回归系数值>0.92。
