Lee Y J, Miyahara T, Noike T
Water Quality Research Department, National Institute of Environmental Research, Seoul, Republic of Korea.
Bioresour Technol. 2001 Dec;80(3):227-31. doi: 10.1016/s0960-8524(01)00067-0.
The effect of the iron concentration in the external environment on hydrogen production was studied using sucrose solution and the mixed microorganisms from a soybean-meal silo. The iron concentration ranged from 0 to 4,000 mg FeCl2 l(-1). The temperature was maintained at 37 degrees C. The maximum specific hydrogen production rate was found to be 24.0 ml g(-1) VSS h(-1) at 4,000 mg FeCl2 l(-1). The specific production rate of butyrate increased with increasing iron concentration from 0 to 20 mg FeCl2 l(-1) and decreased with increasing iron concentration from 20 to 4,000 mg FeCl2 l(-1). The maximum specific production rates of ethanol (682 mg g(-1) VSS h(-1)) and butanol (47.0 mg g(-1) VSS h(-1)) were obtained at iron concentrations of 5 and 3 mg FeCl2 l(-1), respectively. The maximum hydrogen production yield of 131.9 ml g(-1) sucrose was obtained at the iron concentration of 800 mg FeCl2 l(-1). The maximum yields of acetate (389.3 mg g(-1) sucrose), propionate (37.8 mg g(-1) sucrose), and butyrate (196.5 mg g(-1) sucros) were obtained at iron concentrations of 3, 200 and 200 mg FeCl2 l(-1), respectively. The sucrose degradation efficiencies were close to 1.0 when iron concentrations were between 200 and 800 mg FeCl2 l(-1). The maximum biomass production yield was 0.283 g VSS g(-1) sucrose at an iron concentration of 3,000 mg FeCl2 l(-1).
采用蔗糖溶液和来自豆粕筒仓的混合微生物，研究了外部环境中铁浓度对产氢的影响。铁浓度范围为0至4000 mg FeCl₂ l⁻¹。温度保持在37℃。在4000 mg FeCl₂ l⁻¹时，最大比产氢速率为24.0 ml g⁻¹ VSS h⁻¹。丁酸的比产生速率随着铁浓度从0增加到20 mg FeCl₂ l⁻¹而增加，随着铁浓度从20增加到4000 mg FeCl₂ l⁻¹而降低。乙醇（682 mg g⁻¹ VSS h⁻¹）和丁醇（47.0 mg g⁻¹ VSS h⁻¹）的最大比产生速率分别在铁浓度为5和3 mg FeCl₂ l⁻¹时获得。在铁浓度为800 mg FeCl₂ l⁻¹时，获得了131.9 ml g⁻¹蔗糖的最大产氢量。乙酸（389.3 mg g⁻¹蔗糖）、丙酸（37.8 mg g⁻¹蔗糖）和丁酸（196.5 mg g⁻¹蔗糖）的最大产量分别在铁浓度为3、200和200 mg FeCl₂ l⁻¹时获得。当铁浓度在200至800 mg FeCl₂ l⁻¹之间时，蔗糖降解效率接近1.0。在铁浓度为3000 mg FeCl₂ l⁻¹时，最大生物量产率为0.283 g VSS g⁻¹蔗糖。
