Piryazev A P, Azizova O A, Aseichev A V, Dudnik L B, Sergienko V I
Laboratory of Biophysical Bases of Pathology, Research Institute of Physicochemical Medicine, Ministry of Health Care and Social Development, Moscow, Russia.
Bull Exp Biol Med. 2013 Nov;156(1):101-3. doi: 10.1007/s10517-013-2288-9.
We studied the effect of gold nanoparticles on ROS production by leukocytes. ROS production was detected by luminol-dependent chemiluminescence (LDCL) of human peripheral blood leukocytes stimulated with opsonized zymosan. Nanoparticle size was 5, 10 and 30 nm. Simultaneous addition of nanoparticles and opsonized zymosan showed that 5-nm nanoparticles inhibited LDCL intensity in comparison with the control, when LDCL recording was conducted in the presence of opsonized zymosan. Increasing nanoparticle size from 5 up to 30 nm enhanced LDCL intensity. Preincubation of gold nanoparticles with autologous blood plasma increased LDCL intensity. In the control (without gold nanoparticles), blood plasma produced no activating effect on LDCL. We found that the effect of gold nanoparticles on leukocyte LDCL depended on nanoparticle size: 10- and 30-nm nanoparticles inhibited LDCL intensity in comparison with the control (incubation in the absence of nanoparticles) irrespective of the duration of incubation, while 5-nm gold nanoparticles had no effect on LDCL intensity. Incubation of gold nanoparticles with autologous plasma increased LDCL intensity if nanoparticle size was 30 and 10 nm.
我们研究了金纳米颗粒对白细胞产生活性氧（ROS）的影响。通过用调理酵母聚糖刺激的人外周血白细胞的鲁米诺依赖性化学发光（LDCL）来检测ROS的产生。纳米颗粒尺寸为5、10和30纳米。纳米颗粒与调理酵母聚糖同时添加表明，当在调理酵母聚糖存在下进行LDCL记录时，与对照相比，5纳米的纳米颗粒抑制了LDCL强度。将纳米颗粒尺寸从5纳米增加到30纳米可增强LDCL强度。金纳米颗粒与自体血浆预孵育可增加LDCL强度。在对照（无金纳米颗粒）中，血浆对LDCL没有激活作用。我们发现金纳米颗粒对白细胞LDCL的影响取决于纳米颗粒尺寸：与对照（在无纳米颗粒的情况下孵育）相比，10纳米和30纳米的纳米颗粒抑制LDCL强度，与孵育持续时间无关，而5纳米的金纳米颗粒对LDCL强度没有影响。如果纳米颗粒尺寸为30纳米和10纳米，金纳米颗粒与自体血浆孵育可增加LDCL强度。
