用单克隆抗体测定人类风湿因子副蛋白上受限的轻链亚群表达
Restricted light chain subgroup expression on human rheumatoid factor paraproteins determined by monoclonal antibodies.
作者信息
Mageed R A, Walker M R, Jefferis R
出版信息
Immunology. 1986 Nov;59(3):473-8.
Abstract
Two hybridoma antibodies specific for determinants on the V kappa light chain subgroup have been produced and characterized. Antibodies C7 and B12 reacted with distinct V kappa epitopes irrespective of association with heavy chain class or subclass. Epitopes recognized by C7 and B12 were expressed on the light chain of IgG, IgA, and IgM and Bence-Jones paraproteins from the V kappa subgroup. However, a preferential association of both epitopes with IgM RF paraproteins was demonstrated. Hybridomas C7 and B12 reacted with 12/12 and 10/12 IgM RF paraproteins, respectively, but only with 3/6 IgM paraproteins, with no RF activity. Both epitopes C7 and B12, were immunodominant and conformation dependent, being detected by HA, HAI and ELISA on intact light chain but not isolated VK.
摘要
已制备并鉴定了两种针对Vκ轻链亚组决定簇的杂交瘤抗体。抗体C7和B12与不同的Vκ表位反应，而与重链类别或亚类的关联无关。C7和B12识别的表位在来自Vκ亚组的IgG、IgA、IgM和本斯·琼斯副蛋白的轻链上表达。然而，已证明这两个表位与IgM RF副蛋白存在优先关联。杂交瘤C7和B12分别与12/12和10/12 IgM RF副蛋白反应，但仅与3/6无RF活性的IgM副蛋白反应。表位C7和B12均具有免疫显性且依赖构象，可通过HA、HAI和ELISA在完整轻链上检测到，但在分离的VK上未检测到。
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