Gomes Fabio P, Shaw P Nicholas, Whitfield Karen, Koorts Pieter, Hewavitharana Amitha K
School of Pharmacy, The University of Queensland, Queensland 4072, Australia.
Bioanalysis. 2013 Dec;5(24):3063-78. doi: 10.4155/bio.13.283.
The occurrence of vitamin D deficiency has become an issue of serious concern in the worldwide population. As a result numerous analytical methods have been developed, for a variety of matrices, during the last few years to measure vitamin D analogs and metabolites. This review employs a comprehensive search of all vitamin D methods developed during the last 5 years for all applications, using ISI Web of Science(®), Scifinder(®), Science Direct, Scopus and PubMed. Particular emphasis is given to sample-preparation methods and the different forms of vitamin D measured across different fields of applications such as biological fluids, food and pharmaceutical preparations. This review compares and critically evaluates a wide range of approaches and methods, and hence it will enable readers to access developments across a number of applications and to select or develop the optimal analytical method for vitamin D for their particular application.
维生素D缺乏症的出现已成为全球人口中一个严重关切的问题。因此，在过去几年中，针对各种基质开发了许多分析方法来测量维生素D类似物和代谢物。本综述使用ISI Web of Science(®)、Scifinder(®)、Science Direct、Scopus和PubMed，对过去5年开发的适用于所有应用的所有维生素D方法进行了全面检索。特别强调了样品制备方法以及在生物流体、食品和药物制剂等不同应用领域中所测量的维生素D的不同形式。本综述对广泛的方法和途径进行了比较和批判性评估，因此将使读者能够了解多个应用领域的进展情况，并为其特定应用选择或开发用于维生素D的最佳分析方法。
