Kasalová Eva, Aufartová Jana, Krčmová Lenka Kujovská, Solichová Dagmar, Solich Petr
Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic; III. Internal Gerontometabolic Clinic, University Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic.
III. Internal Gerontometabolic Clinic, University Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic.
Food Chem. 2015 Mar 15;171:177-90. doi: 10.1016/j.foodchem.2014.08.102. Epub 2014 Sep 6.
Vitamin D plays an important role in calcium metabolism and affects other metabolic pathways. Despite the intense interest in vitamin D, no comprehensive overview addressing the analysis of vitamin D in milk has been published. Historically, immunoassay techniques have been mainly used for the routine quantification of vitamin D and its metabolites. However, the greater accuracy and precision of chromatography makes it one of the most important methods in the analysis of vitamin D. The determination of vitamin D and its metabolites by LC-MS is the gold standard for its assessment. LC-MS has unique advantages for vitamin D determination and quantification due to its high sensitivity and specificity. In this review, the current status of vitamin D and its metabolites analysis in milk, human and bovine, including sample pre-treatment and chromatography analysis, are critically discussed and summarised.
维生素D在钙代谢中发挥着重要作用，并影响其他代谢途径。尽管人们对维生素D兴趣浓厚，但尚未发表过关于牛奶中维生素D分析的全面综述。从历史上看，免疫分析技术主要用于维生素D及其代谢物的常规定量。然而，色谱法更高的准确性和精密度使其成为维生素D分析中最重要的方法之一。通过液相色谱-质谱联用（LC-MS）测定维生素D及其代谢物是其评估的金标准。由于其高灵敏度和特异性，LC-MS在维生素D的测定和定量方面具有独特优势。在这篇综述中，对牛奶（包括人奶和牛奶）中维生素D及其代谢物分析的现状，包括样品预处理和色谱分析，进行了批判性讨论和总结。
