Sako H, Nakane Y, Okino K, Nishihara K, Kodama M, Kawata M, Yamada H
Histochemistry. 1986;86(1):1-4. doi: 10.1007/BF00492339.
A new double immunohistochemical technique for the simultaneous detection of B-cells and T-cells was investigated, using tissue preparations obtained from human axillary lymph nodes and rejected renal allografts. The specimens were immunostained first for the demonstration of B-cells, by the immunogold-silver staining (IGSS) method using Leu-12 monoclonal antibody, and then for T-cells by the avidin-biotin-peroxidase complex (ABC) method using Leu-1 monoclonal antibody. With the present methods, both B-cells and T-cells were clearly detected and distinctively identified without cross-linking of antibodies or double reaction of enzymes.
利用从人腋窝淋巴结和移植失败的同种异体肾获取的组织标本，研究了一种用于同时检测B细胞和T细胞的新型双重免疫组织化学技术。首先，使用Leu-12单克隆抗体，通过免疫金银染色（IGSS）法对标本进行免疫染色以显示B细胞，然后使用Leu-1单克隆抗体，通过抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法对T细胞进行免疫染色。采用本方法，可清晰检测到B细胞和T细胞，并能对其进行明确鉴别，不存在抗体交联或酶的双重反应。
