Yamada H, Sano Y
Histochemistry. 1985;83(4):285-9. doi: 10.1007/BF00684372.
A newly modified and improved immunohistochemical method was devised for the demonstration of the serotonin neuron system in the central nervous system of the rabbit using serotonin antibody obtained by the immunization of rabbits, i.e., the biotinylation of serotonin antibodies, and the application of the two-step avidin-biotin-peroxidase complex (ABC) method. Using this technique, high background staining and nonspecific reactions were avoided, and extremely clear preparations were produced. The serotonin neurons of rabbit brain, which have a Golgi-like appearance, were followed to the fine terminals. This technique were also applied for electron microscopy, and satisfactory results concerning the submicroscopical distribution of serotonin were obtained.
利用通过兔免疫获得的血清素抗体，即血清素抗体的生物素化，并应用两步抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，设计了一种新的改良免疫组织化学方法，用于显示兔中枢神经系统中的血清素神经元系统。使用该技术，避免了高背景染色和非特异性反应，并制备出极其清晰的标本。具有类似高尔基体外观的兔脑血清素神经元被追踪到其精细终末。该技术也应用于电子显微镜检查，并获得了关于血清素亚显微分布的满意结果。
