Masserdotti Carlo
San Marco Private Veterinary Laboratory, Padua, Italy.
Vet Clin Pathol. 2013 Dec;42(4):522-5. doi: 10.1111/vcp.12086. Epub 2013 Oct 25.
Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the normal proportion of these cells in cytologic specimens from normal livers was not previously established.
The objective of this study was to determine the normal proportion of hepatic mast cells in cytologic specimens from normal canine liver by the use and comparison of the May-Grünwald-Giemsa and the Toluidine Blue staining methods.
The cytologic specimens obtained from fine-needle aspirates of livers of 13 healthy dogs were investigated by counting of hepatocytes and mast cells per high-power field (HPF, x40 objective). The ratio of mast cells/100 hepatocytes was then compared between the 2 staining methods.
A median of 0.17 (range: 0-0.33) mast cells/100 hepatocytes and of 0.47 (range: 0-0.86) mast cells/100 hepatocytes was obtained with May-Grünwald-Giemsa and Toluidine Blue stains, respectively.
Toluidine Blue stain detects more hepatic mast cells than May-Grunwald-Giemsa in normal canine livers. With both stains, < 1% mast cells were detected in normal canine liver samples.
尽管肥大细胞在犬肝脏组织中的存在被认为是正常现象，但此前尚未确定正常肝脏细胞学标本中这些细胞的正常比例。
本研究的目的是通过使用和比较美蓝-姬姆萨染色法和甲苯胺蓝染色法，确定正常犬肝脏细胞学标本中肝肥大细胞的正常比例。
对13只健康犬肝脏细针穿刺获取的细胞学标本进行研究，通过计数每个高倍视野（HPF，40倍物镜）中的肝细胞和肥大细胞数量。然后比较两种染色方法下肥大细胞/100个肝细胞的比例。
美蓝-姬姆萨染色法和甲苯胺蓝染色法分别获得的肥大细胞/100个肝细胞中位数为0.17（范围：0 - 0.33）和0.47（范围：0 - 0.86）。
在正常犬肝脏中，甲苯胺蓝染色法比美蓝-姬姆萨染色法检测到的肝肥大细胞更多。两种染色方法在正常犬肝脏样本中检测到的肥大细胞均<1%。
