Mutsaddi S, Kotrashetti V S, Nayak R S, Pattanshetty S M
Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India.
Biotech Histochem. 2019 Aug;94(6):459-468. doi: 10.1080/10520295.2019.1597986. Epub 2019 Apr 15.
Mast cells are large cells with granular cytoplasm that participate in wound healing, angiogenesis and defense against pathogens. They also contribute to inflammation by initiating innate and acquired immunity. The granules of these cells exhibit characteristic staining properties. We investigated toluidine blue, astra blue, Alcian blue-pyronin Y and May-Grunwald Giemsa stains for mast cells in various oral lesions and assessed the efficacy of each for identifying mast cells. Sections were obtained from 10 each of diagnosed cases of inflammatory fibrous hyperplasia, periapical cyst, mild dysplasia, oral submucous fibrosis and oral squamous cell carcinoma and stained using the stains listed above. Mast cells were assessed for their presence, contrast of the mast cell in the connective tissue background and number. We found that May-Grunwald Giemsa stain was the best for identification of mast cells, although toluidine blue staining is less time-consuming. Overall we obtained better results using May-Grunwald Giemsa and toluidine blue for staining mast cells.
肥大细胞是具有颗粒状细胞质的大细胞，参与伤口愈合、血管生成和抵御病原体。它们还通过启动先天免疫和获得性免疫来促进炎症反应。这些细胞的颗粒具有特征性的染色特性。我们研究了甲苯胺蓝、天青蓝、阿尔辛蓝-派洛宁Y和美-格氏染色法在各种口腔病变中对肥大细胞的染色情况，并评估了每种方法识别肥大细胞的效果。从10例诊断为炎性纤维增生、根尖囊肿、轻度发育异常、口腔黏膜下纤维化和口腔鳞状细胞癌的病例中获取切片，并用上述染色法进行染色。评估肥大细胞的存在情况、结缔组织背景中肥大细胞的对比度以及数量。我们发现美-格氏染色法是识别肥大细胞的最佳方法，尽管甲苯胺蓝染色耗时较少。总体而言，我们使用美-格氏染色法和甲苯胺蓝对肥大细胞进行染色取得了更好的结果。
