牛血清白蛋白自组装对烯丙胺封端硅量子点表面活性剂诱导聚集物稳定性的影响。
Effect of self-association of bovine serum albumin on the stability of surfactant-induced aggregates of allylamine-capped silicon quantum dots.
机构信息
Discipline of Chemistry, Indian Institute of Technology Indore , M-Block, IET-DAVV Campus, Khandwa Road, Indore 452017, M.P., India.
出版信息
J Phys Chem B. 2013 Dec 19;117(50):16110-6. doi: 10.1021/jp4091188. Epub 2013 Dec 10.
The concentration-dependent self-association of bovine serum albumin (BSA) and subsequent altered interaction with sodium dodecyl sulfate (SDS) has been explored by means of photoluminescence (PL) spectroscopy, dynamic light scattering (DLS), circular dichroism (CD), PL imaging, and atomic force microscopy (AFM). By using an extrinsic luminescent probe, allylamine-capped silicon quantum dots (Si-QDs), we have demonstrated the unusual concentration-dependent altered BSA-SDS interaction. Allylamine-capped Si-QDs forms ordered aggregates in the presence of 1 mM SDS due to hydrogen bonding with the surfactants head groups at pH 7.4. Although these aggregates remain stable in the presence of monomeric BSA in the concentration range 1-8 μM, they form typical ring-shaped doughnut-like structures due to "necklace and bead"-like complex formation. However, beyond 10 μM BSA, these aggregates of Si-QDs slowly dissociate and complete dissociation occurs at 150 μM BSA. These anomalous results have been explained by considering the altered hydrophilicity of self-associated BSA.
牛血清白蛋白（BSA）的浓度依赖性自组装以及随后与十二烷基硫酸钠（SDS）的相互作用变化已通过荧光光谱法、动态光散射（DLS）、圆二色性（CD）、荧光成像和原子力显微镜（AFM）进行了研究。通过使用外源性发光探针，丙烯胺封端的硅量子点（Si-QDs），我们已经证明了异常的浓度依赖性改变的 BSA-SDS 相互作用。由于在 pH 值为 7.4 时与表面活性剂的头基形成氢键，丙烯胺封端的 Si-QDs 在 1 mM SDS 的存在下形成有序的聚集。尽管这些聚集物在 1-8 μM 的单体 BSA 存在下保持稳定，但由于“项链和珠子”样的复合物形成，它们形成典型的环形面包圈状结构。然而，超过 10 μM 的 BSA 时，这些 Si-QDs 聚集物缓慢解离，在 150 μM BSA 时完全解离。这些异常结果通过考虑自组装 BSA 的亲水性改变来解释。
Zotero 插件