Răducan Andreea, Mirică Silvia, Duicu Oana, Răducan S, Muntean Danina, Fira-Mlădinescu O, Lighezan Rodica
Department of Pathophysiology, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania;
Rom J Morphol Embryol. 2013;54(3 Suppl):735-9.
Experimental models for the investigation of nerve regeneration are critical in studying new strategies able to promote the repair process. The aim of the present work was to characterize morphological and functional aspects of sciatic nerve regeneration after mechanical crush injury in rodents. Morphological changes were assessed after a four minutes sciatic nerve injury induced by means of a standardized compression clip. Rat nerve samples were collected before injury and after 24 hours, four days, two weeks, and four weeks after injury, respectively. In an additional group with unilateral sciatic nerve injury, animals were evaluated for four weeks using walking track analysis and the sciatic static index (SSI) measured in both rearing and normal standing position. Histological study showed important axonal degeneration at four days and axonal regeneration at four weeks after injury. We observed no significant differences between SSI in rearing and normal standing stance and a strong correlation between SSI values measured in the two positions during the evaluation period. Positive correlations were also found for the footprint parameters. Our data provide a baseline characterization of the sciatic nerve crush injury that will further allow the investigation of peripheral nerve regeneration in the presence of potential neuroprotective agents in post-traumatic nerve repair.
用于研究神经再生的实验模型对于探索能够促进修复过程的新策略至关重要。本研究的目的是描述啮齿动物坐骨神经机械挤压损伤后再生的形态学和功能方面。通过标准化的压迫夹诱导坐骨神经损伤4分钟后评估形态学变化。分别在损伤前、损伤后24小时、4天、2周和4周收集大鼠神经样本。在另一组单侧坐骨神经损伤的动物中，使用行走轨迹分析对动物进行4周评估，并测量其在站立和正常站立姿势下的坐骨神经静态指数（SSI）。组织学研究显示，损伤后4天出现重要的轴突变性，4周出现轴突再生。我们观察到站立和正常站立姿势下的SSI之间无显著差异，且在评估期间两个姿势下测量的SSI值之间存在强相关性。足迹参数之间也发现了正相关。我们的数据提供了坐骨神经挤压损伤的基线特征，这将进一步有助于研究创伤后神经修复中潜在神经保护剂存在时的周围神经再生。
