Bergman Nina, Shevchenko Denys, Bergquist Jonas
Analytical Chemistry, Department of Chemistry - BMC and Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
Anal Bioanal Chem. 2014 Jan;406(1):49-61. doi: 10.1007/s00216-013-7471-3. Epub 2013 Dec 10.
This review summarizes various approaches for the analysis of low molecular weight (LMW) compounds by different laser desorption/ionization mass spectrometry techniques (LDI-MS). It is common to use an agent to assist the ionization, and small molecules are normally difficult to analyze by, e.g., matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) using the common matrices available today, because the latter are generally small organic compounds themselves. This often results in severe suppression of analyte peaks, or interference of the matrix and analyte signals in the low mass region. However, intrinsic properties of several LDI techniques such as high sensitivity, low sample consumption, high tolerance towards salts and solid particles, and rapid analysis have stimulated scientists to develop methods to circumvent matrix-related issues in the analysis of LMW molecules. Recent developments within this field as well as historical considerations and future prospects are presented in this review.
本综述总结了通过不同激光解吸/电离质谱技术（LDI-MS）分析低分子量（LMW）化合物的各种方法。使用一种试剂辅助电离是常见的做法，小分子通常难以通过例如使用当今常见基质的基质辅助激光解吸/电离质谱（MALDI-MS）进行分析，因为后者本身通常是小有机化合物。这常常导致分析物峰的严重抑制，或者在低质量区域中基质和分析物信号的干扰。然而，几种LDI技术的固有特性，如高灵敏度、低样品消耗、对盐和固体颗粒的高耐受性以及快速分析，促使科学家们开发方法来规避在分析LMW分子时与基质相关的问题。本综述介绍了该领域的最新进展以及历史考量和未来前景。
