State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China.
J Gen Virol. 2014 Mar;95(Pt 3):712-718. doi: 10.1099/vir.0.059626-0. Epub 2013 Dec 9.
In this study, sequences of small RNA (sRNA) libraries derived from the insect vector Laodelphax striatellus were assembled into contigs and used as queries for database searches. A large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi P virus (HiPV). Interestingly, HiPV-derived sRNAs had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. HiPV sRNAs had a strong bias towards the sense strand, whilst the antisense sRNAs were predominantly 21 and 22 nt. HiPV sRNAs do not have the typical features of PIWI-interacting RNAs, but their 3' ends were preferentially cleaved at UA-rich sequences. Our data suggest that HiPV sRNAs may be derived both from activities of the RNA interference pathway and from cleavage of the viral genome by other host RNases.
在这项研究中，从小RNA (sRNA)文库中获得的昆虫载体褐飞虱的序列被组装成连续体，并用作数据库搜索的查询。大量的连续体与昆虫双 RNA 病毒——Himetobi P 病毒 (HiPV) 的基因组序列高度同源。有趣的是，HiPV 衍生的 sRNA 具有广泛的大小分布，在整个 18-30nt 大小范围内相对丰富，只有在 22nt 处略有峰值。HiPV sRNA 对正义链有很强的偏向性，而反义 sRNA 主要是 21 和 22nt。HiPV sRNA 没有 PIWI 相互作用 RNA 的典型特征，但它们的 3' 端在富含 UA 的序列处被优先切割。我们的数据表明，HiPV sRNA 可能既来源于 RNA 干扰途径的活性，也来源于其他宿主核糖核酸酶对病毒基因组的切割。
