Chen Meng-Nan, Ye Zhuang-Xin, Feng Ke-Hui, Yuan Jing-Na, Chen Jian-Ping, Zhang Chuan-Xi, Li Jun-Min, Mao Qian-Zhuo
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Insects. 2024 Aug 15;15(8):615. doi: 10.3390/insects15080615.
Herbivorous insects harbor a variety of insect-specific viruses (ISVs) some of which are considered to be valuable biological agents for potential applications in biological defense and control strategies. Leaf beetles with chewing mouthparts are particularly known for their capacity to disrupt plant tissue while feeding, often creating openings that can act as entry points for plant pathogens. In this study, we have identified two new negative-sense RNA viruses infecting the leaf beetle , an important member of the Chrysomelidae family. These recently discovered viruses belong to the viral families and and have been preliminarily named nyami-like virus 1 (AINlV1) and chu-like virus 1 (AIClV1), respectively. The complete genomic sequences of these viruses were obtained using rapid amplification of cDNA ends (RACE) techniques. Detailed analysis of their genomic structures has confirmed their similarity to other members within their respective families. Furthermore, analysis of virus-derived small interfering RNA (vsiRNA) demonstrated a high abundance and typical vsiRNA pattern of AINlV1 and AIClV1, offering substantial evidence to support their classification as ISVs. This research enhances our understanding of viral diversity within insects.
植食性昆虫携带多种昆虫特异性病毒（ISVs），其中一些被认为是有价值的生物制剂，可用于生物防御和控制策略。具有咀嚼式口器的叶甲虫尤其以其取食时破坏植物组织的能力而闻名，它们常常造成开口，这些开口可成为植物病原体的侵入点。在本研究中，我们鉴定出两种感染叶甲虫的新型负链RNA病毒，叶甲虫是叶甲科的重要成员。这些最近发现的病毒分别属于病毒科 和 ，并已分别初步命名为尼亚米样病毒1（AINlV1）和楚样病毒1（AIClV1）。使用cDNA末端快速扩增（RACE）技术获得了这些病毒的完整基因组序列。对其基因组结构的详细分析证实了它们与各自科内其他成员的相似性。此外，对病毒衍生的小干扰RNA（vsiRNA）的分析表明，AINlV1和AIClV1具有高丰度和典型的vsiRNA模式，为支持将它们归类为ISVs提供了大量证据。这项研究增进了我们对昆虫体内病毒多样性的理解。
