Department of Neurosurgery, Hokkaido University Graduate School of Medicine, North 15, West 7, Kita-ku, Sapporo, 060-8638, Japan.
Transl Stroke Res. 2012 Mar;3(1):99-106. doi: 10.1007/s12975-011-0138-5. Epub 2011 Dec 13.
Recent studies have elucidated that transplantation of the bone marrow stromal cells (BMSC) has therapeutic potential for the central nervous system (CNS) disorders. However, no imaging modalities have been established to track the engrafted cells in the CNS in clinical situation. This study aimed to investigate the ability of magnetic resonance imaging (MRI) to visualize the BMSC labeled with superparamagnetic iron oxide (SPIO). The BMSC of mice were labeled with SPIO. Various numbers of the cells were injected into the agar phantom and were visualized using a 3.0-T MR apparatus. The SPIO-labeled cells were injected into the temperature-sensitive gelation polymer (TGP) hydrogel and were cultured for 7 days. They were also visualized just after the injection and at 7 days postinjection. After a 7-day culture, they were stained with Turnbull blue technique. T2-, T2*-, and susceptibility-weighted imaging could identify minimally 1,000 cells in the agar or TGP hydrogel, although it was difficult to quantify their number on MRI. All of these sequences could track the SPIO-labeled BMSC for at least 7 days when injected into the TGP. Turnbull blue staining revealed the survival and proliferation of the SPIO-labeled BMSC in the TGP for 7 days. The findings strongly suggest that the SPIO labeling may enable to track minimally 1,000 cells engrafted in the CNS on clinical MR apparatus. These data would be valuable to consider the application of imaging technique into cell transplantation therapy for CNS disorders.
最近的研究表明，骨髓基质细胞（BMSC）移植对于中枢神经系统（CNS）疾病具有治疗潜力。然而，在临床情况下，还没有建立用于追踪 CNS 中移植细胞的成像方式。本研究旨在探讨磁共振成像（MRI）可视化超顺磁性氧化铁（SPIO）标记的 BMSC 的能力。用 SPIO 标记小鼠的 BMSC。将不同数量的细胞注入琼脂体模中，并使用 3.0-T MR 设备进行可视化。将 SPIO 标记的细胞注入温度敏感凝胶化聚合物（TGP）水凝胶中，并培养 7 天。在注射后即刻和 7 天后也对其进行了可视化。培养 7 天后，用特伦伯蓝技术对其进行染色。T2-、T2*-和磁化率加权成像可以在琼脂或 TGP 水凝胶中识别至少 1000 个细胞，尽管在 MRI 上很难对其数量进行定量。当注入 TGP 时，所有这些序列都可以至少在 7 天内追踪到 SPIO 标记的 BMSC。特伦伯蓝染色显示 SPIO 标记的 BMSC 在 TGP 中存活和增殖至少 7 天。这些发现强烈表明，SPIO 标记可以在临床 MRI 设备上追踪到 CNS 中植入的至少 1000 个细胞。这些数据对于考虑将成像技术应用于 CNS 疾病的细胞移植治疗将具有重要意义。
