催产素和环磷酸腺苷通过蟾蜍膀胱顶端膜中对钙离子敏感、对氨氯吡脒不敏感的通道刺激单价阳离子运动。
Oxytocin and cAMP stimulate monovalent cation movements through a Ca2+-sensitive, amiloride-insensitive channel in the apical membrane of toad urinary bladder.
作者信息
Van Driessche W, Aelvoet I, Erlij D
出版信息
Proc Natl Acad Sci U S A. 1987 Jan;84(1):313-7. doi: 10.1073/pnas.84.1.313.
Abstract
The effects of oxytocin and cAMP on ion transport were investigated in toad urinary bladders incubated with Ca2+-free solutions on the apical side. Under these conditions both oxytocin and cAMP markedly stimulated the movements of Na+, K+, Rb+, Cs+, Li+, and NH4+ through a pathway that is insensitive to amiloride. The amiloride-insensitive currents were inhibited by the addition of Ca2+, Sr2+, or Mg2+ to the apical solution. The movement of the monovalent cations was associated with a spontaneous Lorentzian component in the power spectrum of the fluctuation in short-circuit current. The plateau of the Lorentzian component was enhanced by oxytocin and cAMP and was depressed by divalent cations. Methohexital inhibited the stimulation of monovalent cation movements caused by oxytocin. These findings suggest that oxytocin and cAMP activate at least two kinds of ionic channels in the apical membrane of toad urinary bladder: the well-known amiloride-sensitive channel and an amiloride-insensitive channel that allows the movement of several monovalent cations and is blocked by Ca2+ and other divalent cations.
摘要
在顶端侧用无钙溶液孵育的蟾蜍膀胱中，研究了催产素和环磷酸腺苷（cAMP）对离子转运的影响。在这些条件下，催产素和cAMP均通过对氨氯吡脒不敏感的途径显著刺激了Na⁺、K⁺、Rb⁺、Cs⁺、Li⁺和NH₄⁺的转运。向顶端溶液中添加Ca²⁺、Sr²⁺或Mg²⁺可抑制氨氯吡脒不敏感电流。单价阳离子的转运与短路电流波动功率谱中的自发洛伦兹分量相关。洛伦兹分量的平台期在催产素和cAMP作用下增强，而在二价阳离子作用下降低。美索比妥抑制了催产素引起的单价阳离子转运刺激。这些发现表明，催产素和cAMP激活了蟾蜍膀胱顶端膜中的至少两种离子通道：著名的氨氯吡脒敏感通道和一种氨氯吡脒不敏感通道，该通道允许几种单价阳离子转运，并被Ca²⁺和其他二价阳离子阻断。
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