Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, the Fourth Military Medical University, Xi'an, China.
Diagn Pathol. 2013 Dec 10;8:200. doi: 10.1186/1746-1596-8-200.
MicroRNA-21 has been proved to be associated with glioma proliferation and invasion; thus, we sought to clarify the clinical value of miR-21 expression in glioma tissues with WHO grade I to IV.
One hundred and fifty-two pairs of human gliomas and non-neoplastic brain tissues were evaluated using real-time PCR. The association of miR-21 expression with clinicopathological factors or the prognosis of glioma patients was also analyzed. In this study, survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model.
MiR-21 was more greatly expressed in glioma tissues compared to the corresponding non-neoplastic brain tissues (P < 0.001). This observed high miR-21 expression was significantly associated with high pathological grades and the Karnofsky performance score of glioma patients. In addition, overall patient survival for those with low miR-21 expression was significantly longer than those patients with high miR-21 expression (P < 0.001). Moreover, multivariate Cox regression analysis indicated that miR-21 might be an independent prognostic marker for glioma patient survival.
Our data show that miR-21 may be a candidate independent marker for gliomas, especially those with high pathological grades, and this could also be a potential therapeutic target for molecular glioma therapy.
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已有研究证实 microRNA-21 与脑胶质瘤的增殖和侵袭有关；因此，我们旨在明确 WHO 分级 I-IV 的脑胶质瘤组织中 miR-21 表达的临床价值。
采用实时 PCR 技术检测 152 对人脑胶质瘤组织及其配对的非瘤脑组织中 miR-21 的表达情况，分析 miR-21 表达与脑胶质瘤患者临床病理因素及预后的关系。本研究采用 Kaplan-Meier 法和 Cox 比例风险回归模型进行生存分析。
与相应的非瘤脑组织相比，脑胶质瘤组织中 miR-21 表达显著增高（P<0.001）。miR-21 的高表达与高病理分级和患者的 Karnofsky 行为状态评分显著相关。此外，miR-21 低表达患者的总生存时间显著长于 miR-21 高表达患者（P<0.001）。多因素 Cox 回归分析表明，miR-21 可能是脑胶质瘤患者生存的独立预后标志物。
本研究数据表明，miR-21 可能是脑胶质瘤的独立候选标志物，尤其是高病理分级的脑胶质瘤，可能成为胶质瘤分子治疗的潜在靶点。
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