Wilson-Gerwing Tracy D, Pratt Isaac V, Cooper David M L, Silver Tawni I, Rosenberg Alan M
Department of Pediatrics, University of Saskatchewan, Saskatoon, Canada.
Comp Med. 2013;63(6):498-502.
Arthritis is among the most common chronic diseases in both children and adults. Although intraarticular inflammation is the feature common among all patients with chronic arthritis there are, in addition to age at onset, clinical characteristics that further distinguish the disease in pediatric and adult populations. In this study, we aimed to demonstrate the utility of microCT (μCT) and ultrasonography in characterizing pathologic age-related differences in a collagen-induced arthritis (CIA) rat model. Juvenile (35 d old) and young adult (91 d old) male Wistar rats were immunized with bovine type II collagen and incomplete Freund adjuvant to induce polyarthritis. Naïve male Wistar rats served as controls. All paws were scored on a scale of 0 (normal paw) to 4 (disuse of paw). Rats were euthanized at 14 d after the onset of arthritis and the hindpaws imaged by μCT and ultrasonography. Young adult rats had more severe signs of arthritis than did their juvenile counterparts. Imaging demonstrated that young adult CIA rats exhibited more widespread and severe skeletal lesions of the phalanges, metatarsals, and tarsal bones, whereas juvenile CIA rats had more localized and less proliferative and osteolytic damage that was confined predominantly to the phalanges and metatarsals. This report demonstrates the utility of imaging modalities to compare juvenile and young adult rats with CIA and provides evidence that disease characteristics and progression differ between the 2 age groups. Our observations indicate that the CIA model could help discern age-related pathologic processes in inflammatory joint diseases.
关节炎是儿童和成人中最常见的慢性病之一。尽管关节内炎症是所有慢性关节炎患者共有的特征，但除了发病年龄外，还有一些临床特征可进一步区分儿童和成人人群中的这种疾病。在本研究中，我们旨在证明微计算机断层扫描（μCT）和超声检查在表征胶原诱导性关节炎（CIA）大鼠模型中与年龄相关的病理差异方面的效用。将幼年（35日龄）和年轻成年（91日龄）雄性Wistar大鼠用牛II型胶原和不完全弗氏佐剂免疫以诱导多关节炎。未免疫的雄性Wistar大鼠作为对照。所有爪子按0（正常爪子）至4（爪子废用）的评分标准进行评分。在关节炎发作后14天对大鼠实施安乐死，并通过μCT和超声检查对后爪进行成像。年轻成年大鼠的关节炎症状比幼年大鼠更严重。成像显示，年轻成年CIA大鼠的指骨、跖骨和跗骨出现更广泛、更严重的骨骼病变，而幼年CIA大鼠的损伤更局限，增殖性和溶骨性损伤更少，主要局限于指骨和跖骨。本报告证明了成像方式在比较幼年和年轻成年CIA大鼠方面的效用，并提供了证据表明两个年龄组之间的疾病特征和进展有所不同。我们的观察结果表明，CIA模型有助于识别炎症性关节疾病中与年龄相关的病理过程。
