Department of Materials Chemistry and Chemical Technology, Institute of Chemistry, University of Silesia, 40-006 Katowice, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Mar 25;122:451-60. doi: 10.1016/j.saa.2013.11.061. Epub 2013 Nov 21.
The interactions between etoposide, cytarabine and 1,2-dihexadecanoyl-sn-glycerol-3-phosphocholine bilayers were studied using differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR). These techniques have proven to be a very powerful tool in studying the structure and dynamics of phospholipid bilayers. In particular, DSC can provide information on the phase transition temperature and cooperativity of the lipid molecules in the absence and presence of the drug. Vibrational spectroscopy is well suited to the study of drug-lipid interactions, since it allows for an investigation of the conformation of phospholipid molecules at different levels in lipid bilayers and follows structural changes that occur during the gel to liquid-crystalline phase transition. NMR supported the determination of the main phase transition temperatures (TC) of 1,2-dihexadecanoyl-sn-glycerol-3-phosphocholine (DPPC). The main phase transition temperature (TC) determined by (1)H NMR is comparable with values obtained by DSC for all studied liposomes. The location of cytarabine and etoposide in liposomes was also determined by NMR. Atomic force microscopy (AFM) images, acquired immediately after sample deposition on a mica surface, revealed the spherical shape of lipid vesicles.
使用差示扫描量热法（DSC）、傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）研究了依托泊苷、阿糖胞苷和 1,2-二硬脂酰-sn-甘油-3-磷酸胆碱双层之间的相互作用。这些技术已被证明是研究磷脂双层结构和动力学的非常有力的工具。特别是，DSC 可以提供有关脂质分子在没有和存在药物时的相变温度和协同性的信息。振动光谱非常适合研究药物-脂质相互作用，因为它允许在不同水平的脂质双层中研究磷脂分子的构象，并跟踪凝胶到液晶相转变过程中发生的结构变化。NMR 支持确定 1,2-二硬脂酰-sn-甘油-3-磷酸胆碱（DPPC）的主要相变温度（TC）。（1）H NMR 确定的主要相变温度（TC）与所有研究的脂质体的 DSC 获得的值相当。通过 NMR 还确定了阿糖胞苷和依托泊苷在脂质体中的位置。在云母表面上沉积样品后立即获得的原子力显微镜（AFM）图像显示了脂质囊泡的球形。
