从昆虫病原真菌白僵菌中分离到一种新型victo 病毒的分子特征。
Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria bassiana.
机构信息
Department of Molecular Bioscience, College of Biomedical Science, Kang Won National University, Hyoja Dong, Chun Cheon Si, Kangwon Do, Korea.
出版信息
Arch Virol. 2014 Jun;159(6):1321-7. doi: 10.1007/s00705-013-1938-6. Epub 2013 Dec 11.
New Zealand isolates of the entomopathogenic fungus Beauveria were examined for the presence of dsRNAs and virus-like particles. Seven out of nine isolates contained one or more high-molecular-weight dsRNAs and all seven contained isometric virus particles ranging in size from 30 to 50 nm. B. bassiana isolate ICMP#6887 contained a single dsRNA band of ~6 kb and isometric virus-like particles of ~50 nm in diameter. Sequencing revealed that the virus from ICMP#6887 had a genome of 5,327 nt with two overlapping ORFs coding for a putative coat protein (CP) and an RNA-dependent RNA-polymerase (RdRp). The sequence showed a highest CP identity of 58.3 % to Tolypocladium cylindrosporum virus 1 (TcV1) and a highest RdRp identity of 48.8 % to Sphaeropsis sapinea RNA virus 1 (SsRV1). Since both TcV1 and SsRV1 belong to the genus Victorivirus, the new virus from B. bassiana ICMP#6887 was tentatively assigned the name Beauveria bassiana victorivirus 1 (BbVV1-6887).
新西兰的虫生真菌白僵菌分离株被检测是否存在双链 RNA 和类病毒颗粒。在 9 个分离株中，有 7 个含有一个或多个高分子量双链 RNA，且这 7 个分离株都含有等径病毒颗粒，大小在 30 到 50nm 之间。白僵菌 ICMP#6887 分离株含有一条大小约为 6kb 的单链 RNA 带和大小约为 50nm 的等径病毒样颗粒。测序结果显示，ICMP#6887 中的病毒基因组全长 5327nt，含有两个重叠的开放阅读框，分别编码一个假定的外壳蛋白 (CP) 和 RNA 依赖性 RNA 聚合酶 (RdRp)。该序列的 CP 与 Tolypocladium cylindrosporum virus 1 (TcV1) 的同源性最高，为 58.3%，RdRp 与 Sphaeropsis sapinea RNA virus 1 (SsRV1) 的同源性最高，为 48.8%。由于 TcV1 和 SsRV1 都属于 Victorivirus 属，因此，白僵菌 ICMP#6887 中的新病毒被暂时命名为白僵菌 Victorivirus 1 (BbVV1-6887)。
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