Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Gongzhuling, Jilin, China.
College of Life Sciences, Jilin Normal University, Siping, China.
Virulence. 2024 Dec;15(1):2401978. doi: 10.1080/21505594.2024.2401978. Epub 2024 Sep 12.
Mycoviruses can alter the biological characteristics of host fungi, including change virulence or pathogenicity of phytopathogens and entomopathogenic fungi (EPF). However, most studies on the mycoviruses found in EPF have focused on the effects of the viruses on the virulence of host fungi towards insect pests, with relatively few reports on the effects to the host fungi with regard to plant disease resistance in hosts. The present study investigated the effects of the mycovirus chrysovirus 2 (BbCV2) virus infection on host biological characteristics, evaluated antagonistic activity of BbCV2 against two phytopathogenic fungi ( and ), and transcriptome analysis was used to reveal the interactions between viruses and hosts. Our results showed that BbCV2 virus infection increased 's growth rate, spore production, and biomass, it also enhanced the capacity of host fungi and their metabolic products to inhibit phytopathogenic fungi. BbCV2 virus infection reduced the contents of the two pathogens in tomato plants significantly, and transcriptome analysis revealed that the genes related to competition for ecological niches and nutrition, mycoparasitism and secondary metabolites in were significantly up-regulated after viral infection. These findings indicated that the mycovirus infection is an important factor to enhance the ability of against plant disease after endophytic colonization. We suggest that mycovirus infection causes a positive effect on against phytopathogens, which should be considered as a potential strategy to promote the plant disease resistance of EPF.
真菌病毒可以改变宿主真菌的生物学特性，包括改变植物病原菌和昆虫病原真菌（EPF）的毒力或致病性。然而，大多数关于 EPF 中发现的真菌病毒的研究都集中在病毒对宿主真菌对昆虫害虫的毒力的影响上，而关于病毒对宿主真菌在宿主中对植物病害抗性的影响的报道相对较少。本研究调查了真菌病毒 Chrysovirus 2（BbCV2）感染对宿主生物学特性的影响，评估了 BbCV2 对两种植物病原菌（和）的拮抗活性，并进行了转录组分析以揭示病毒与宿主之间的相互作用。我们的结果表明，BbCV2 病毒感染增加了宿主真菌的生长速度、孢子产生和生物量，也增强了宿主真菌及其代谢产物抑制植物病原菌的能力。BbCV2 病毒感染显著降低了番茄植株中两种病原菌的含量，转录组分析显示，病毒感染后与竞争生态位和营养物质、真菌寄生和次生代谢物相关的基因在中显著上调。这些发现表明，真菌病毒感染是内生菌定植后增强对植物病害能力的一个重要因素。我们建议，真菌病毒感染对 EPF 对抗植物病原菌产生积极影响，这应被视为促进 EPF 植物抗病性的潜在策略。
