Hutson P H, Sarna G S, Sahakian B J, Dourish C T, Curzon G
Ann N Y Acad Sci. 1986;473:321-36. doi: 10.1111/j.1749-6632.1986.tb23626.x.
The techniques of repeated CSF withdrawal and intracerebral dialysis are described. They were used to monitor 5HT metabolism in the brains of freely moving rats. Evidence indicates that results obtained proportionately reflect changes of 5HT metabolism in whole brain (CSF method) and in the striatum (intracerebral dialysis). The potential value of the methods is illustrated by two behavioral studies. The first suggested that 5HT turnover values (determined two weeks prior to behavioral testing by the CSF method) were predictive for neck + body biting in a social interaction test. The second study showed a consistent pattern of change of hypothalamic dialysate 5HIAA concentration associated with feeding in rats on a restricted food intake schedule.
本文描述了重复脑脊液抽取和脑内透析技术。这些技术用于监测自由活动大鼠大脑中的5-羟色胺（5HT）代谢。有证据表明，所获得的结果能成比例地反映全脑（脑脊液方法）和纹状体（脑内透析）中5HT代谢的变化。两项行为学研究说明了这些方法的潜在价值。第一项研究表明，（通过脑脊液方法在行为测试前两周测定的）5HT周转率值可预测社交互动测试中的咬颈+咬身体行为。第二项研究显示，在限制食物摄入时间表的大鼠中，下丘脑透析液5-羟吲哚乙酸（5HIAA）浓度的变化模式与进食一致。
