Anderson G M, Teff K L, Young S N
Life Sci. 1987 Jun 8;40(23):2253-60. doi: 10.1016/0024-3205(87)90061-0.
A simple, selective reverse-phase HPLC-fluorometric method is described for the determination of serotonin (5HT) in cisternal CSF of the rat. The mean (+/- SE) value of CSF 5HT observed in control adult rats was 457 +/- 83 pg/ml (N = 16). In an attempt to validate the measure as an index of extracellular, or functionally active, 5HT, groups of animals were treated with fenfluramine, amitriptyline, pargyline, pargyline plus tryptophan, and 5-hydroxytryptophan plus carbidopa. In all cases CSF 5HT appeared to reflect well the presumed effects of the agents on extracellular levels of 5HT. CSF 5HT was superior in this regard to brain 5HT, brain 5HIAA, or CSF 5HIAA levels. The measurement of cisternal CSF 5HT would appear to offer a convenient index of functionally active 5HT.
本文描述了一种简单、选择性的反相高效液相色谱-荧光法，用于测定大鼠脑池脑脊液中的5-羟色胺（5HT）。在成年对照大鼠中观察到的脑脊液5HT平均（±标准误）值为457±83 pg/ml（N = 16）。为了验证该测量方法作为细胞外或功能活性5HT指标的有效性，对几组动物分别用芬氟拉明、阿米替林、帕吉林、帕吉林加色氨酸以及5-羟色氨酸加卡比多巴进行处理。在所有情况下，脑脊液5HT似乎都能很好地反映这些药物对5HT细胞外水平的假定影响。在这方面，脑脊液5HT优于脑5HT、脑5-羟吲哚乙酸（5HIAA）或脑脊液5HIAA水平。脑池脑脊液5HT的测量似乎提供了一个方便的功能活性5HT指标。
