miR-1 和 PIK3CA 在非小细胞肺癌中的表达水平与临床特征和预后的相关性。
Correlation between the expression levels of miR-1 and PIK3CA in non-small-cell lung cancer and their relationship with clinical characteristics and prognosis.
机构信息
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Road, Nanjing 210029, Jiangsu, People's Republic of China.
出版信息
Future Oncol. 2014 Jan;10(1):49-57. doi: 10.2217/fon.13.242.
AIMS
To examine the relationship between the expression levels of miR-1 and PIK3CA in resected non-small-cell lung cancer (NSCLC) tissues and clinical characteristics and prognosis.
MATERIALS & METHODS: Levels of miR-1 and PIK3CA in cancerous and normal tissues obtained from 55 patients with NSCLC were measured by real-time PCR and western blot assays, and their associations with clinicopathological features were evaluated.
RESULTS
A total of 69.1% of the NSCLC tissue samples showed low miR-1 expression (p < 0.05) and 70.9% showed high PIK3CA expression (p < 0.05). Patients with low miR-1 expression and those with high PIK3CA expression had significantly higher incidences of lymph node metastases and postoperative recurrences within 1 year after surgery than those with moderate miR-1 expression and moderate PIK3CA expression (p < 0.05 for both).
CONCLUSION
Low expression of miR-1 and overexpression of PIK3CA in NSCLC tissues may be useful predictors of lymph node metastasis and postoperative recurrence in patients with NSCLC.
目的
检测 miR-1 和 PIK3CA 在切除的非小细胞肺癌 (NSCLC) 组织中的表达水平与临床特征和预后之间的关系。
材料与方法
通过实时 PCR 和 Western blot 检测 55 例 NSCLC 患者癌组织和正常组织中的 miR-1 和 PIK3CA 水平，并评估其与临床病理特征的相关性。
结果
共 69.1%的 NSCLC 组织样本显示 miR-1 表达较低（p<0.05），70.9%的样本显示 PIK3CA 表达较高（p<0.05）。miR-1 低表达和 PIK3CA 高表达的患者淋巴结转移和术后 1 年内复发的发生率显著高于 miR-1 中表达和 PIK3CA 中表达的患者（p<0.05）。
结论
NSCLC 组织中 miR-1 的低表达和 PIK3CA 的过表达可能是 NSCLC 患者淋巴结转移和术后复发的有用预测指标。
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