Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Road, Nanjing 210029, Jiangsu, People's Republic of China.
Future Oncol. 2014 Jan;10(1):49-57. doi: 10.2217/fon.13.242.
To examine the relationship between the expression levels of miR-1 and PIK3CA in resected non-small-cell lung cancer (NSCLC) tissues and clinical characteristics and prognosis.
MATERIALS & METHODS: Levels of miR-1 and PIK3CA in cancerous and normal tissues obtained from 55 patients with NSCLC were measured by real-time PCR and western blot assays, and their associations with clinicopathological features were evaluated.
A total of 69.1% of the NSCLC tissue samples showed low miR-1 expression (p < 0.05) and 70.9% showed high PIK3CA expression (p < 0.05). Patients with low miR-1 expression and those with high PIK3CA expression had significantly higher incidences of lymph node metastases and postoperative recurrences within 1 year after surgery than those with moderate miR-1 expression and moderate PIK3CA expression (p < 0.05 for both).
Low expression of miR-1 and overexpression of PIK3CA in NSCLC tissues may be useful predictors of lymph node metastasis and postoperative recurrence in patients with NSCLC.
检测 miR-1 和 PIK3CA 在切除的非小细胞肺癌 (NSCLC) 组织中的表达水平与临床特征和预后之间的关系。
通过实时 PCR 和 Western blot 检测 55 例 NSCLC 患者癌组织和正常组织中的 miR-1 和 PIK3CA 水平，并评估其与临床病理特征的相关性。
共 69.1%的 NSCLC 组织样本显示 miR-1 表达较低（p<0.05），70.9%的样本显示 PIK3CA 表达较高（p<0.05）。miR-1 低表达和 PIK3CA 高表达的患者淋巴结转移和术后 1 年内复发的发生率显著高于 miR-1 中表达和 PIK3CA 中表达的患者（p<0.05）。
NSCLC 组织中 miR-1 的低表达和 PIK3CA 的过表达可能是 NSCLC 患者淋巴结转移和术后复发的有用预测指标。
