Zhao Lei, Liu Guo-Hua, Zhao Guang-Hui, Cai Jin-Zhong, Zhu Xing-Quan, Qian Ai-Dong
College of Animal Science and Technology, Jilin Agricultural University , Changchun, Jilin Province , P.R. China .
Mitochondrial DNA. 2015 Apr;26(2):167-70. doi: 10.3109/19401736.2013.843089. Epub 2013 Dec 11.
This study examined sequence differences in mitochondrial cytochrome c oxidase subunit 1 (cox1), large subunit ribosomal RNA (rrnL) and NADH dehydrogenase subunits 1 and 4 (nad1 and nad4) between Chabertia ovina and C. erschowi from yaks in Qinghai and goats in Shaanxi provinces, China. A part of the cox1 (pcox1), rrnL (prrnL), nad1 and nad4 genes (pnad1 and pnad4) were amplified separately from individual nematodes by PCR and sequenced. The length of the sequences of pcox1, prrnL, pnad1 and pnad4 was 441 bp, 450 bp, 526 bp and 914 bp for C. ovina, and 441 bp, 451 bp, 517 bp and 810 bp for C. erschowi, respectively. The intra-specific sequence variations within C. ovina were 0.2-2.9% for pcox1, 0-0.9% for prrnL, 0.6-2.3% for pnad1, and 0.4-2.0% for pnad4, and were 0.5-1.6% for pcox1, 0-1.1% for prrnL, 0.2-1.7% for pnad1, and 0.4-1.1% for pnad4 within C. erschowi. Whereas, the inter-specific sequence differences between the two species were obviously higher, being 11.6-12.9% for pcox1, 9.8-11.1% for prrnL, 14.4-15.9% for pnad1, and 16.4-17.7% for pnad4. Phylogenetic analyses using Bayesian inference (BI), based on combined sequences of four genes, indicated that the C. ovina and C. erschowi represent distinct species. These results demonstrate that these mt gene sequences provide novel genetic markers for the identificaiton and differentiation C. ovina and C. erschowi, and have implications for studying the population genetics and molecular epidemiology of Chabertia spp.
本研究检测了来自中国青海省牦牛的绵羊夏伯特线虫（Chabertia ovina）和陕西省山羊的叶氏夏伯特线虫（C. erschowi）之间线粒体细胞色素c氧化酶亚基1（cox1）、核糖体大亚基RNA（rrnL）以及NADH脱氢酶亚基1和4（nad1和nad4）的序列差异。通过PCR分别从单个线虫中扩增出cox1的一部分（pcox1）、rrnL（prrnL）、nad1和nad4基因（pnad1和pnad4）并进行测序。绵羊夏伯特线虫的pcox1、prrnL、pnad1和pnad4序列长度分别为441 bp、450 bp、526 bp和914 bp，叶氏夏伯特线虫的分别为441 bp、451 bp、517 bp和810 bp。绵羊夏伯特线虫种内pcox1的序列变异为0.2 - 2.9%，prrnL为0 - 0.9%，pnad1为0.6 - 2.3%，pnad4为0.4 - 2.0%；叶氏夏伯特线虫种内pcox1的序列变异为0.5 - 1.6%，prrnL为0 - 1.1%，pnad1为0.2 - 1.7%，pnad4为0.4 - 1.1%。然而，两个物种之间的种间序列差异明显更高，pcox1为11.6 - 12.9%，prrnL为9.8 - 11.1%，pnad1为14.4 - 15.9%，pnad4为16.4 - 17.7%。基于四个基因的联合序列，使用贝叶斯推断（BI）进行系统发育分析表明，绵羊夏伯特线虫和叶氏夏伯特线虫代表不同物种。这些结果表明，这些线粒体基因序列为绵羊夏伯特线虫和叶氏夏伯特线虫的鉴定和区分提供了新的遗传标记，并对研究夏伯特属线虫的群体遗传学和分子流行病学具有重要意义。
