Sie P, Ofosu F, Fernandez F, Buchanan M R, Petitou M, Boneu B
Br J Haematol. 1986 Dec;64(4):707-14. doi: 10.1111/j.1365-2141.1986.tb02232.x.
The in vitro anticoagulant effects of standard heparin (SH) and of seven other sulphated polysaccharides (SPS) were investigated by measuring activated partial thromboplastin time (APTT) prolongation of normal plasma and of plasmas selectively depleted of antithrombin III (AT III), of heparin cofactor II (HC II) and of both heparin cofactors. This allowed the determination of the relative contribution of each of the two heparin cofactors to the SPS anticoagulant effect. The SPS varied in their relative activities as catalysts of thrombin inhibition by purified AT III or HC II. The anticoagulant activities of heparin and dermatan sulphate were primarily attributable to their ability to enhance thrombin inhibition by AT III and HC II respectively. Heparin had an additional minor anticoagulant activity which was independent of both AT III and HC II. Pentosan polysulphate, high molecular weight dextran sulphate, heparin with low affinity for AT III and a sulphated heparin derivative had weaker anticoagulant activities in normal plasma than standard heparin. The anticoagulant activities of these last four SPS in plasma depleted of both AT III and HC II were similar to their respective activities in normal plasma. This suggests that these SPS act by directly preventing thrombin generation rather than by enhancing thrombin inhibition.
通过测量正常血浆以及选择性去除抗凝血酶III（AT III）、肝素辅因子II（HC II）和两种肝素辅因子的血浆的活化部分凝血活酶时间（APTT）延长情况，研究了标准肝素（SH）和其他七种硫酸化多糖（SPS）的体外抗凝作用。这使得能够确定两种肝素辅因子各自对SPS抗凝作用的相对贡献。SPS作为纯化的AT III或HC II抑制凝血酶的催化剂，其相对活性各不相同。肝素和硫酸皮肤素的抗凝活性主要分别归因于它们增强AT III和HC II抑制凝血酶的能力。肝素还有一种额外的次要抗凝活性，该活性独立于AT III和HC II。在正常血浆中，戊聚糖多硫酸盐、高分子量硫酸葡聚糖、对AT III低亲和力的肝素以及一种硫酸化肝素衍生物的抗凝活性比标准肝素弱。这四种SPS在同时去除AT III和HC II的血浆中的抗凝活性与其在正常血浆中的各自活性相似。这表明这些SPS通过直接阻止凝血酶生成起作用，而不是通过增强凝血酶抑制作用。
