鉴定肝素辅因子II为戊聚糖多硫酸盐（SP54）抗凝血酶活性的主要血浆辅因子。
Identification of heparin cofactor II as the principal plasma cofactor for the antithrombin activity of pentosan polysulphate (SP54).
作者信息
Scully M F, Kakkar V V
出版信息
Thromb Res. 1984 Oct 15;36(2):187-94. doi: 10.1016/0049-3848(84)90340-2.
In antithrombin III depleted plasma only a slight reduction was observed in thrombin inhibitory activity due to potentiation by dermatan sulphate or by pentosan polysulphate (SP54). On gel filtration of normal plasma the peak of pentosan polysulphate dependent activity eluted with a higher apparent molecular weight than that observed with low concentrations of heparin. During purification of heparin cofactor II (1) good coincidence was observed between yields of its activity and the pentosan polysulphate dependent inhibitory activity and cochromatography of the two activities was observed also. Maximal second order rate constant of 2.5 X 10(8) M-1 min-1 (with apparent Kd of 1.8 microM) was found for the potentiation of the thrombin/heparin cofactor II interaction by pentosan polysulphate. A polysaccharide of high charge density may therefore potentiate heparin cofactor II activity and this may be of importance in vivo.
在抗凝血酶III缺乏的血浆中，由于硫酸皮肤素或聚硫酸戊聚糖（SP54）的增强作用，凝血酶抑制活性仅略有降低。对正常血浆进行凝胶过滤时，聚硫酸戊聚糖依赖性活性的峰值以比低浓度肝素更高的表观分子量洗脱。在肝素辅因子II的纯化过程中，观察到其活性产量与聚硫酸戊聚糖依赖性抑制活性之间有良好的一致性，并且还观察到两种活性的共色谱分离。聚硫酸戊聚糖对凝血酶/肝素辅因子II相互作用的增强作用的最大二级速率常数为2.5×10⁸ M⁻¹ min⁻¹（表观解离常数为1.8 μM）。因此，高电荷密度的多糖可能会增强肝素辅因子II的活性，这在体内可能很重要。
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